基于^(13)C标记的低磷胁迫杉木根系亲缘识别分析  

Analysis of root kin recognition ability of Chinese fir in low phosphorus environments based on ^(13)C labeling

作　　者：李亚超 王讷敏 张慧 林文奖 马祥庆[1,2] 吴鹏飞 LI Yachao;WANG Nemin;ZHANG Hui;LIN Wenjiang;MA Xiangqing;WU Pengfei(College of Forestry,Fujian Agriculture and Forestry University,Fuzhou 350002,China;National Forestry Grassland Engineering and Technology Research Center of Chinese Fir,Fuzhou 350002,China;Yancheng Branch,Jiangsu Bureau of Hydrology and Water Resources Survey,Yancheng 224001,China;Mawei Minjiang Estuary Wetland Provincial Nature Reserve Management Office,Fuzhou 350002,China;Wuyi State-owned Forest Farm,Zhangping,Fujian Province,Longyan 364400,China)

机构地区：[1]福建农林大学林学院,福州350002 [2]国家林业草原杉木工程技术研究中心,福州350002 [3]江苏省水文水资源勘测局盐城分局,盐城224001 [4]福建省马尾闽江河口湿地省级自然保护区管理处,福州350002 [5]福建省漳平五一国有林场,龙岩364400

出　　处：《生态学报》2024年第23期10827-10837,共11页Acta Ecologica Sinica

基　　金：国家自然科学基金项目(31870614);福建农林大学科技创新专项基金项目(KFB23051)。

摘　　要：为了探究低磷(P)胁迫下不同亲缘关系邻株对杉木生长的影响规律,验证杉木根系是否具有亲缘识别行为,采用室内盆栽控制试验,选择2个杉木半同胞家系(No.32、No.28)为试验材料,利用^(13)C同位素标记方法,分别在不同供P水平条件下(1.0 mmol/L KH_(2)PO_(4),P1和0 mmol/L KH_(2)PO_(4),P0),构建亲缘组(No.32+No.32^(*),^(*)为进行^(13)C同位素标记的植株)、非亲缘组(No.28+No.32^(*))2种亲缘邻株栽植模式,以杉木单株栽植(No.32^(*))为对照处理(CK),分别对不同处理中No.32杉木幼苗的地上部进行^(13)C同位素标记,测定不同亲缘邻株下的杉木根长、根表面积、根体积、根平均直径等生长指标,以及邻株参试苗木叶、茎、根的^(13)C丰度的差异性。No.32^(*)的邻株植物在未标记^(13)C的情况下检测出^(13)C丰度发生显著变化。在不供P处理下发现非亲缘组No.28的全株总^(13)C丰度显著高于亲缘组No.32,表明低P胁迫促进杉木与非亲缘邻株的C交流。不供P处理下非亲缘组No.32^(*)杉木全株生物量比亲缘组No.32^(*)低了31.3%,供P水平显著改变了杉木与邻株的相处生长模式,与非亲缘植株相邻的杉木全株生物量减少,根冠比降低,生长发育减弱。不供P处理下,非亲缘组No.32^(*)比CK组提高46.0%根表面积,降低74.7%根体积。不供P处理下,非亲缘邻株促进杉木根表面积增加,提高P获取能力,增强杉木地下部分竞争力。不供P处理下,非亲缘组No.32^(*)全株P含量与CK组没有显著差异,而亲缘组显著低于CK组,非亲缘组No.32^(*)全株P利用效率(PUE)显著低于亲缘组,可见,在低P胁迫下亲缘邻株可促进杉木PUE的提高,缓解杉木的低P胁迫,维持逆境中杉木的正常生长发育。本研究发现杉木与邻株存在基于^(13)C交流的亲缘识别,低P胁迫下杉木面对亲缘植物表现出提高相互响应度,提高PUE,面对非亲缘植物则表现出较强竞争力。养分匮乏下,杉木面对不同的亲�To investigate the impact of kinship on the growth of Chinese fir under conditions of low phosphorus(P)availability and determine if kin recognition behavior exists Chinese fir roots,an indoor controlled pot experiment was conducted.Two half-sib families(No.32 and No.28)of Chinese fir were selected as the experimental subjects.Using the^(13)C isotope labeling technique,plants from both the kin(No.32+No.32^(*),^(*)labeled with^(13)C isotope)and non-kin(No.28+No.32^(*))groups were established under different levels of P supply(1.0 mmol/L KH_(2)PO_(4),P1 and 0 mmol/L KH_(2)PO_(4),P0).A single planting of Chinese fir(No.32^(*))served as the control(CK).In the different treatments,the aboveground parts of Chinese fir(No.32^(*))seedlings were isotopically labeled with^(13)C.Growth parameters,including root length,surface area,volume,and average diameter,were measured for Chinese fir seedlings under different kinship neighboring trees.Additionally,the differential patterns in^(13)C abundance were determined for roots,stems,and leaves of neighboring seedlings of No.32^(*).The^(13)C abundance in neighboring plants of different kin of No.32^(*)exhibited significant heterogeneity.Under P-deficient conditions,the^(13)C abundance in the non-kin group(No.28)was significantly higher than in the kin group(No.32),indicating that low P stress facilitates carbon exchange between unrelated individuals of Chinese fir.Conversely,under low P stress,the total biomass of the non-kin group(No.32^(*))was 31.3%lower than that of the kin group.Varying levels of P supply significantly altered the growth pattern of Chinese fir and its neighboring plants.The total biomass of Chinese fir adjacent to non-kin neighboring plants decreased,accompanied by a reduction in root-to-shoot ratio and weakened growth and development.Under low P stress,the non-kin group(No.32^(*))exhibited a 46.0%increase in root surface area compared to the CK group(No.32^(*)),while experiencing a 74.7%decrease in root volume.Under low P stress,non-kin neighboring plants pro

关 键 词：杉木 根系亲缘识别 ^(13)C同位素标记 低磷胁迫 邻株竞争 

分 类 号：S791.27[农业科学—林木遗传育种]