抗酶解抗菌肽的研究进展  

Research progress in anti-enzymatic antimicrobial peptides

作　　者：邵长轩 王梦成 王袁梦雪 何诗琪 朱永杰 单安山[1] SHAO Changxuan;WANG Mengcheng;WANG Yuanmengxue;HE Shiqi;ZHU Yongjie;SHAN Anshan(College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,Heilongjiang,China)

机构地区：[1]东北农业大学动物科学技术学院,黑龙江哈尔滨150030

出　　处：《生物工程学报》2024年第12期4396-4407,共12页Chinese Journal of Biotechnology

基　　金：中国博士后科学基金(2022M720694);黑龙江省自然科学基金优秀青年项目(YQ2022C015);黑龙江省博士后资助项目(LBH-Z22007);黑龙江省高校协同创新成果项目(LJGXCG2022-022)。

摘　　要：抗菌肽(antimicrobial peptides,AMPs)是机体先天免疫中的一类小分子多肽,作为机体防御的第一道防线,在自然界中广泛存在。AMPs具有多种生物学活性且不易产生耐药性,但天然AMPs容易被体内的各种消化酶降解。在AMPs中引入非天然氨基酸、化学修饰、合理规避酶切位点进行氨基酸重排、肽的环化、纳米肽设计等提高AMPs蛋白酶稳定的方法不断出现。本文重点综述了提高AMPs蛋白酶稳定性的各种方法,以期为此领域的研究提供参考。Antimicrobial peptides(AMPs)are small molecular peptides widely existing in the innate immunity of organisms,serving as the first line of defense.Natural AMPs possess various biological activities and are difficult to develop drug resistance.However,they are easily broken down by digestive enzymes in the body.In recent years,increasing methods have been reported to enhance the stability of AMPs,including incorporation of unnatural amino acids,chemical modifications,strategic avoidance of enzyme cleavage sites,cyclization,and nano peptide design.This review summarizes the methods for improving the stability of AMPs against protease degradation,aiming to provide references for further research in this field.

关 键 词：抗菌肽 蛋白酶稳定性 非天然氨基酸 化学修饰 残基排布 环化 纳米技术 

分 类 号：Q511[生物学—生物化学]