生长因子对出芽短梗霉产聚苹果酸的影响  

Effect of Growth Factors on Poly Malic Acid Production by Aureobasidium pullulans

作　　者：李清 王淑贤 韩璐瑶 王蕊 赵廷彬 张琳 曹伟锋[1,3,4] 乔长晟 LI Qing;WANG Shuxian;HAN Luyao;WANG Rui;ZHAO Tingbin;ZHANG Lin;CAO Weifeng;QIAO Changsheng(College of Bioengineering,Tianjin University of Science and Technology,Tianjin 300457,China;Tianjin Huizhi Baichuan Bioengineering Co.,Ltd.,Tianjin 300457,China;Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education and Tianjin Key Laboratory of Industrial Microbiology,Tianjin 300457,China;Tianjin Engineering Center of Microbial Metabolism and Fermentation Process)

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]天津慧智百川生物有限公司,天津300457 [3]工业发酵微生物教育部重点实验室暨天津市工业微生物重点实验室,天津300457 [4]天津市微生物代谢与发酵过程控制技术工程中心,天津300457

出　　处：《食品研究与开发》2024年第24期27-34,共8页Food Research and Development

基　　金：国家自然科学基金项目(22278412);宁夏回族自治区重点研发计划项目(2021BEG03011、2022BEG02006)。

摘　　要：聚苹果酸是一种天然可生物降解的聚合物,在医学及其它工业中的应用愈加受到重视,但其生物合成的产量仍然较低。该文主要研究生长因子(胞嘧啶、腺嘌呤、维生素H、吡哆醇和氯化胆碱)对聚苹果酸合成的影响,并揭示其影响机制。研究发现,5种生长因子中腺嘌呤对出芽短梗霉产聚苹果酸的影响最大,其较适宜的添加浓度为0.5 g/L。添加0.5 g/L腺嘌呤时聚苹果酸产量从29.4 g/L提高到39.3 g/L,产量提高33.7%。利用非标记定量技术和液相色谱‐串联质谱法分析腺嘌呤对聚苹果酸合成的影响机制。与空白组相比,添加0.5 g/L腺嘌呤出现398个差异蛋白质,其中202个上调蛋白,196个下调蛋白(差异倍数>1.2,P<0.05)。对差异蛋白进行基因本体(gene ontology,GO)功能富集、(kyoto encyclopedia of genes and genomes,KEGG)通路富集分析和代谢途径分析,结果显示差异蛋白质主要参与糖酵解和三羧酸循环(tricarboxylic acid cycle,TCA)途径。As a naturally biodegradable polymer,poly malic acid(PMLA)has attracted more and more atten‐tion in medicine and other industries.However,PMLA remains low in its biosynthetic yield.This paper studied the effect of growth factors,including cytosine,adenine,vitamin H,pyridoxine,and choline chloride,on the synthesis of PMLA and the underlying mechanisms were revealed.It was found that among the five growth fac‐tors,adenine had the greatest effect on PMLA production by Aureobasidium pullulans,with an optimal concen‐tration of 0.5 g/L.By adding 0.5 g/L adenine,the PMLA production went up from 29.4 g/L to 39.3 g/L,an in‐crease of 33.7%.Further,the label‐free quantitative method and liquid chromatography‐tandem mass spectrom‐etry were used to analyze the mechanism of adenine's influence on PMLA synthesis.Compared with the control group,the addition of 0.5 g/L adenine resulted in 398 differential proteins,including 202 up‐regulated proteins and 196 down‐regulated proteins(fold change>1.2,P<0.05).Through gene ontology(GO)functional enrich‐ment analysis,KEGG pathway enrichment analysis and metabolic pathway analysis of the differential proteins,it could be seen that the differential proteins were mainly involved in glycolysis and tricarboxylic acid cycle(TCA)cycle pathways.

关 键 词：腺嘌呤 出芽短梗霉 聚苹果酸 液相色谱‐串联质谱法 蛋白质组学 

分 类 号：TQ317[化学工程—高聚物工业] TQ920.1[轻工技术与工程—发酵工程]