贵州小型猪2型糖尿病气阴两虚证模型建立及评价  

Establishment and evaluation of a Qi Yin deficiency syndrome model of type 2 diabetes in Guizhou miniature pigs

作　　者：邱华明 李子祥 唐杰 吴延军 赵海 吴曙光 QIU Huaming;LI Zixiang;TANG Jie;WU Yanjun;ZHAO Hai;WU Shuguang(Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China)

机构地区：[1]贵州中医药大学,贵阳550025

出　　处：《中国实验动物学报》2024年第11期1399-1407,共9页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(82160957);贵州省科技计划项目(黔科合支撑[2021]一般148),贵州省科技计划项目(黔科合基础-ZK[2024]一般360)。

摘　　要：目的本研究旨在探讨贵州小型猪2型糖尿病(type 2 diabetes mellitus,T2DM)气阴两虚证模型的构建方法及评价体系和相关指标。方法选取6月龄贵州小型猪26只,其中正常组6只采用基础饲料喂养10个月,T2DM气阴两虚证模型组20只采用高糖高脂饲料饲喂10个月、青皮附子水煎剂喂养3个月,构建了贵州小型猪T2DM气阴两虚证模型。以小便情况、大便情况、行动、精神、对周围环境反应及皮毛鼻盘色泽5方面量表评价中医证型,血清CD4^(+)、CD8^(+)及两者比值,cAMP、cGMP及两者比值、舌象红色值(R)、绿色值(G)和蓝色值(B)评估气阴两虚证。采用空腹血糖(fasting blood glucose,FBG)、空腹胰岛素(fasting insulin,FINS)、糖化血红蛋白(glycated hemoglobin,HbA1c)、糖耐量实验(glucose tolerance test,OGTT)、胰岛素抵抗指数(homeostasis model assessment of insulin resistance,HOMA-IR)、甘油三酯(triglycerides,TG)、总胆固醇(total cholesterol,TC)、高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C)、凝血酶原时间(prothrombin time,PT)、活化部分凝血活酶时间(activated partial thromboplastin time,APTT)等指标变化,苏木素-伊红(HE)染色观察胰腺组织形态学变化来评价T2DM。结果模型组小型猪的体征可概括为体型偏胖、神疲乏力、好卧、懒动、精神萎靡、无神、食少多饮、皮毛无光泽、皮毛灰暗;二便、行动及精神、对周围环境反应、皮毛鼻盘色泽等评分偏低(P<0.05);舌象呈舌红无苔或少苔;与正常组相比,模型组小型猪实验室指标出现了FBG、HbA1c、HOMA-IR、TC、LDL-C、cAMP、cGMP、舌象R、G、B值显著降低,PT、APTT、CD4^(+)、CD4^(+)/CD8^(+)值显著下降,TG升高(P>0.05),HDL-C升高(P>0.05),cGMP升高(P>0.05);病理染色显示胰腺组织破坏。结论采用高糖高脂饲料配合破气伤阴中药饲喂的方法可成功建立T2DM气阴两虚小型Objective To explore the construction method,evaluation system,and related indicators of the Qi Yin deficiency syndrome model of type 2 diabetes(T2DM)in Guizhou miniature pigs.Methods Twenty-six 6-month-old Guizhou miniature pigs were divided into 2 groups:pigs fed a basic diet for 10 months(normal group,n=6),and pigs fed a high-sugar,high-fat diet for 10 months followed by Qingpi Fuzi decoction for 3 months(Qi Yin deficiency in T2DM model group,n=20).Qi Yin deficiency in the T2DM model was created in Guizhou miniature pigs.Traditional Chinese medicine syndrome types were evaluated using five scales,including urination,defecation,movement and mental state,response to the surrounding environment,and color of the skin,hair,and nasal disc.Serum CD4^(+)and CD8^(+)and their ratios,cAMP and cGMP and their ratios,as well as tongue RGB color values were used to evaluate Qi Yin deficiency syndrome.Fasting blood glucose(FBG),glycosylated hemoglobin(HbA1c),glucose tolerance test(OGTT),homeostasis model assessment of insulin resistance(HOMA-IR),triglycerides(TG),total cholesterol(TC),high-density lipoprotein-cholesterol(HDL-C),low-density lipoprotein-cholesterol(LDL-C),prothrombin time(PT),activated partial thromboplastin time(APTT),and other indicators were used to evaluate T2DM.Results Physically,the model group pigs were overweight and showed fatigue,with a tendency to lie down,lethargy,overeating and drinking,and dull fur.The defecation,movement and mental state,response to the surrounding environment,and color of the skin,fur,and nasal disc scores were relatively low(P<0.05).The tongue appeared red with no or little coating.Pigs in the model group showed significantly lower of FBG,HbA1c,HOMA-IR,TC,LDL-C,cAMP,and cGMP,compared with the normal group.The RGB values of the tongue images were significantly reduced.The PT,APTT,CD4^(+),and CD4^(+)/CD8^(+)values were also significantly reduced,and TG,HDL-C,and cGMP were all elevated(all P>0.05).Pathological staining showed destruction of pancreatic tissue.Conclusions The method

关 键 词：贵州小型猪 2型糖尿病 气阴两虚证 空腹血糖 

分 类 号：Q95-33[生物学—动物学]