CRISPR-Cas13-mediated RNA editing in the silkworm Bombyx mori  

作　　者：Yao-Hao Tang Xing Zhang Zong-Cai Dai Hao Li Yan Yang Tu-Jing Zhao Dong-Qin Yuan Wen-Liang Qian Dao-Jun Cheng 

机构地区：[1]Integrative Science Center of Germplasm Creation in Western China(CHONGQING)Science City,Biological Science Research Center,Southwest University,Chongqing 400715,China [2]State Key Laboratory of Resource Insects,Southwest University,Chongqing 400715,China

出　　处：《Zoological Research》2024年第6期1249-1260,共12页动物学研究（英文）

基　　金：supported by the National Natural Science Foundation of China(32070496,32370555);Fundamental Research Funds for the Central Universities(SWU120033);Technology Innovation and Application Development Program of Chongqing(CSTB2024TIADKPX0023)。

摘　　要：The CRISPR-Cas13 system,an RNA-guided editing tool,has emerged as a highly efficient and stable RNA editing technique.Although the CRISPR-Cas13 system has been developed in several insect species,its application in lepidopterans has not yet been reported.In the present study,we evaluated the RNA cleavage activity of the CRISPR-Cas13 system in the silkworm(Bombyx mori),a model lepidopteran insect,both ex vivo and in vivo.We established two stable silkworm BmE cell lines expressing PspCas13b and CasRx,respectively.Further analysis demonstrated that both PspCas13b and CasRx effectively down-regulated the transcription of exogenouslyintroduced target and endogenous genes in these cell lines.In addition,we generated two transgenic silkworm strains,one expressing CasRx and the other expressing RNA-guided CRISPR RNA targeting Sex combs reduced(Scr).Further crossing experiments showed that CasRx induced a down-regulation of Scr transcription in silkworms,which impaired systemic growth of larvae.Overall,this study demonstrated that the CRISPR-Cas13RNA editing system works efficiently in the silkworm,providing a potential alternative approach for RNA manipulation in lepidopteran insects.

关 键 词：SILKWORM CRISPR PspCas13b CasRx RNA editing 

分 类 号：Q78[生物学—分子生物学]