Peptide Cy_(RL-QN15)accelerates hair regeneration in diabetic mice by binding to the Frizzled-7 receptor  

作　　者：Yu-Tong Wu Ze-Qiong Ru Ying Peng Zhe Fu Qiu-Ye Jia Zi-Jian Kang Yuan-Sheng Li Yu-Bing Huang Sai-Ge Yin Kun Guo Nai-Xin Liu Cheng-An Feng Jing Tang Bai-Yu Zhang Zhi Yang Ying Wang Xin-Wang Yang 

机构地区：[1]Department of Anatomy and Histology&Embryology,Faculty of Basic Medical Science,Kunming Medical University,Kunming,Yunnan 650500,China [2]Key Laboratory of Chemistry in Ethnic Medicinal Resources&Key Laboratory of Natural Products Synthetic Biology of Ethnic Medicinal Endophytes,State Ethnic Affairs Commission&Ministry of Education,School of Ethnic Medicine,Yunnan Minzu University,Kunming,Yunnan 650504,China [3]Department of Biochemistry and Molecular Biology,Faculty of Basic Medical Science,Kunming Medical University,Kunming,Yunnan 650500,China [4]Department of Urology,First Affiliated Hospital of Kunming Medical University,Kunming,Yunnan 650500,China [5]Dermatology,First Affiliated Hospital of Kunming Medical University,Kunming,Yunnan 650500,China [6]Yunnan Key Laboratory of Pharmacology for Natural Products,Kunming Medical University,Kunming,Yunnan 650500,China

出　　处：《Zoological Research》2024年第6期1287-1299,共13页动物学研究（英文）

基　　金：supported by the Key Program of Yunnan Applied Basic Research Project(202301AS070036);Outstanding Youth Program of Yunnan Applied Basic Research Project-Kunming Medical University Union Foundation(202301AY070001-301);First-Class Discipline Team of Skin&Mucosal Regenerative Medicine of Kunming Medical University(2024XKTDTS10);National Natural Science Foundation of China(32360138,32301054,32060212,82371567);Program of Yunnan Applied Basic Research Project-Kunming Medical University Union Foundation(202301AY070001-012,202101AY070001-006,202301AY070001-198,202101AY070001-035,202101AY070001-036,202401AY070001-028,202401AY070001-068)。

摘　　要：Individuals with diabetes frequently face serious challenges,including delayed wound healing and increased risk of infection.Notably,the regeneration of hair follicles plays a crucial role in accelerating diabetic skin damage repair,reducing the risk of infection,and enhancing overall skin health.Research has predominantly emphasized the re-epithelialization of diabetic wounds,with a paucity of in-depth studies on hair follicle regeneration.In the current study,we explored the effects of a bioactive amphibian-derived peptide,Cy_(RL-QN15),on promoting hair regeneration in a diabetic skin model.In vivo experiments demonstrated that local treatment with Cy_(RL-QN15)not only accelerated wound healing of scalded skin on the backs of diabetic Kunming(KM)mice but also improved growth of damaged hair follicles.Additionally,back-shaved diabetic C57BL/6 mice showed a significant increase in the growth of newly formed hair after 28 days of continuous Cy_(RL-QN15)treatment.Further analysis indicated that the hairregenerating effects of Cy_(RL-QN15)were closely associated with the proliferation and migration of hair follicle stem cells(HFSCs).Cy_(RL-QN15)enhanced intracellularβ-catenin expression by binding to the Frizzled-7 receptor on the surface of HFSCs.The up-regulation inβ-catenin modulated the levels of downstream proteins,such as cMYC,Cyclin D1,and Lef1,ultimately inducing hair regeneration.This study not only reveals the robust effects of the bioactive peptide Cy_(RL-QN15)in hair follicle regeneration but also provides novel avenues for the development of more targeted and effective therapeutics for diabetic wound healing in the future.

关 键 词：CyRL-QN15 Hair follicle stem cells(HFSCs) Β-CATENIN Frizzled-7 

分 类 号：R587.1[医药卫生—内分泌] R-332[医药卫生—内科学]