Investigation of sex determination in starry flounder(Platichthys stellatus)reveals sex chromosome evolution in Pleuronectiformes and identifies a sex-specific marker  

作　　者：Wei-Jing Li Yu-Man Zhang Shuo Li Yu-Yan Liu Chen Li Sheng-Lei Han Kai-Qiang Liu Lucas Benites Doretto Bing-Hua Liu Hai-Jun Huang Zhi-Long Sun Qian Wang Hong-Yan Wang Chang-Wei Shao 

机构地区：[1]State Key Laboratory of Mariculture Biobreeding and Sustainable Goods,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao,Shandong 266071,China [2]School of Graduate,Chinese Academy of Agricultural Sciences,Beijing 100081,China [3]Laboratory for Marine Fisheries Science and Food Production Processes,Qingdao Marine Science and Technology Center,Qingdao,Shandong 266237,China

出　　处：《Zoological Research》2024年第6期1347-1356,共10页动物学研究（英文）

基　　金：supported by the National Key Research and Development Program of China(2022YFD2400100);National Natural Science Foundation of Shandong Province of China(ZR2023QC006);National Natural Science Foundation of China(32403053)。

摘　　要：The identification of sex chromosomes is fundamental for exploring the mechanism and evolution of sex determination.Platichthys stellatus,a species exhibiting clear sexual dimorphism and homomorphic chromosome pairs,has received limited research concerning its sex determination mechanisms.Clarifying the sex chromosome of P.stellatus will enhance our understanding of sex chromosome evolution in Pleuronectiformes.This study employed whole-genome resequencing to investigate the sex chromosome and sex determination system in P.stellatus.Notably,Chr23 was identified as the sex chromosome in P.stellatus,with the sex-determining region(SDR)occupying 48.1%of the chromosome and featuring an XX/XY system.Sex chromosome turnover was observed within Pleuronectiformes,with P.stellatus,Verasper variegatus,and Hippoglossus hippoglossus sharing a common ancestral karyotype.No inversions were detected within the SDR of P.stellatus,although chromosomal rearrangements between sex chromosomes and autosomes were identified.Additionally,a sex-specific marker for P.stellatus was ascertained,enabling genetic sex identification,with significant implications for improving breeding programs and aquaculture practices.

关 键 词：Sex chromosome Platichthys stellatus Sex determination system Ancestral karyotype of Pleuronectiformes Sex-specific marker 

分 类 号：Q953[生物学—动物学]