和肝汤颗粒通过调控转录因子蛋白家族核因子κB信号途径改善功能性消化不良十二指肠微炎症  

Hegantang Granules improve duodenal low-grade inflammation of functional dyspepsia by regulating NF-κB signaling pathway

作　　者：赵静怡 郑金粟[1] 王梓淞 陆梦馨 曹锐[1] ZHAO Jingyi;ZHENG Jinsu;WANG Zisong;LU Mengxin;CAO Rui(Department of Traditional Chinese Medicine,Beijing Chao-Yang Hospital,Capital Medicine University,Beijing 100020,China)

机构地区：[1]首都医科大学附属北京朝阳医院中医科,北京100020

出　　处：《环球中医药》2024年第12期2418-2425,共8页Global Traditional Chinese Medicine

基　　金：北京市医管局培育计划(PZ2022003、PZ2024003);首都医科大学附属北京朝阳医院金种子科研基金(CYJZ202344)。

摘　　要：目的探索和肝汤改善功能性消化不良(functional dyspepsia,FD)十二指肠微炎症的机制。方法8只6周龄大鼠随机分为正常组、模型组、和肝汤组,每组6只。模型组、和肝汤组大鼠以夹尾应激法建立FD模型,造模后和肝汤组予临床等效剂量和肝汤颗粒剂灌胃,取大鼠胃、十二指肠行苏木精—伊红染色、甲苯胺蓝染色、嗜酸染色检测组织形态和炎症细胞聚集情况,实时荧光定量PCR、酶联免疫吸附法检测多种促炎、抗炎因子的表达水平,通过实时荧光定量PCR、蛋白免疫印记、免疫荧光检测转录因子蛋白家族核因子κB(nuclear factor kappa-B,NF-κB)P65的表达和激活水平。结果模型组较正常组其肥大细胞、嗜酸性粒细胞聚集更为显著(P<0.01),促炎因子和抗炎因子表达增多(P<0.01),NF-κB P65和p-P65在十二指肠的表达上调(P<0.01),和肝汤治疗后炎症因子含量和炎症细胞聚集减少(P<0.01),NF-κB P65和p-P65蛋白表达水平下调(P<0.05,P<0.01)。结论FD十二指肠微炎症状态下,NF-κB信号途径被部分激活,促炎—抗炎平衡被打破,而和肝汤治疗可下调NF-κB P65的表达和激活水平,恢复抗炎因子和促炎因子的平衡,这可能是和肝汤改善FD十二指肠微炎症的部分分子生物学机制。Objective To explore the mechanism of Hegantang in improving duodenal low-grade inflammation in functional dyspepsia(FD).Methods 186-week-old rats were randomly divided into the normal control,FD and Hegantang group,with 6 rats in each group.The rats in the FD group and the Hegantang group were given tail-clip stimulation method.The Hegantang group was given clinical equivalent dose of Hegantang granules by gavage.The stomach and duodenum of the rats were taken for hematoxylin-eosin staining,toluidine blue staining and eosinophilic staining to detect the tissue morphology and the aggregation of inflammatory cells.The expression of inflammatory factors,NF-κB P65 and p-P65 were detected by the methods of qPCR,ELISA,Western blot and immunofluorescence.Results Compared with the normal control group,the aggregation of mast cells and eosinophils in the FD group was more significant(P<0.01),the expression of pro-inflammatory factor and anti-inflammatory factor was increased(P<0.01),and the expression level of NF-κB P65 and p-P65 in duodenum were up-regulated(P<0.01).After Hegantang treatment,the aggregation of inflammatory cells and the expression of inflammatory factors were decreased(P<0.01),and the content of P65,p-P65 were down-regulated(P<0.05,P<0.01).Conclusion In the condition of FD duodenal microinflammation,NF-κB signaling pathway is partially activated along with the imbalance of pro-inflammation and anti-inflammation.Hegantang can down-regulate the expression and activation of NF-κB P65 and restore the balance of anti-inflammatory and pro-inflammatory factors,which may be part of the molecular biological mechanism of Hegantang to improve FD duodenal low-grade inflammation.

关 键 词：和肝汤 方和谦 功能性消化不良 炎症因子 转录因子蛋白家族核因子κB信号通路 十二指肠微炎症 

分 类 号：R285.5[医药卫生—中药学]