探究单次肝区推拿对小鼠急性酒精性肝损伤的影响  

Exploring the effect of single abdominal massage on acute alcoholic liver injury in mice

作　　者：林小娟 陈幼楠[1] 杨昌铭 刘晓栋 缪林清 LIN Xiaojuan;CHEN Younan;YANG Changming;LIU Xiaodong;MIAO Linqing(School of Acupuncture-Moxibustion and Tuina,Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学针灸推拿学院,100029 [2]北京理工大学机电学院 [3]北京理工大学智能机器人与系统高精尖创新中心

出　　处：《环球中医药》2024年第12期2434-2440,共7页Global Traditional Chinese Medicine

基　　金：国家自然科学基金(81803856)。

摘　　要：目的探究单次肝区推拿对急性酒精性肝损伤小鼠的影响。方法24只8周龄C57BL/6雄性小鼠,随机分为空白组、模型组、6小时推拿组和24小时推拿组,每组6只。通过两次间隔12小时灌胃75%乙醇(每次12μL/g)建立急性酒精性肝损伤模型。在造模后对6小时推拿组和24小时推拿组进行5分钟单次肝区推拿。根据干预后取材时间点不同,分别在6小时(模型组、空白组和6小时推拿组)和24小时(24小时推拿组)后取材。计算小鼠体质量和肝脏质量的比值;检测小鼠血清中丙氨酸氨基转移酶(alanine aminotransferase,ALT)、天冬氨酸氨基转移酶(aspartate aminotransferase,AST)、甘油三酯(triglyceride,TG)、高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C)以及小鼠肝匀浆中超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽(glutathione,GSH)、丙二醛(malondialdehyde,MDA)的水平;采用苏木素—伊红(hematoxylin-eosin,HE)染色法观察肝组织形态;油红染色观察肝细胞脂肪变性。结果(1)肝脏指数、AST、ALT:与空白组相比,模型组肝脏指数、ALT、AST升高(P<0.05)。与模型组相比,6小时推拿组ALT、AST降低(P<0.05)。24小时推拿组肝脏指数、ALT、AST降低(P<0.05)。(2)HE染色:模型组肝细胞显示肝索排列混乱,肝小叶结构受损。6小时推拿组和24小时推拿组的肝细胞病变数量显著减少,肝索排列更为规整。(3)TG、HDL-C、LDL-C:与空白组相比,模型组的TG升高(P<0.05),HDL-C降低(P<0.05)。与模型组相比,6小时推拿组的TG降低(P<0.05),HDL-C升高(P<0.05);24小时推拿组的TG、LDL-C降低(P<0.05),HDL-C升高(P<0.05)。(4)油红染色:模型组肝细胞中脂滴增多,6小时推拿组和24小时推拿组的脂肪变性程度明显减轻。(5)GSH、MDA、SOD:与空白组相比,模型组的GSH降低(P<0.05),SOD升高(P<0.05)。与模型组相比,6小时推拿组和24小时推拿组的GSObjective This study aims to explore the effects of a single session of hepatic area massage on mice with acute alcoholic liver injury.Methods Twenty-four 8-week-old male C57BL/6 mice were randomly divided into four groups:the control group,the model group,the 6-hour massage group,and the 24-hour massage group,with six mice in each group.Acute alcoholic liver injury was induced by administering 75%ethanol via gavage at a dose of 12μL/g twice,with a 12-hour interval between administrations.After the model was established,the mice in the 6-hour and 24-hour massage groups received a single 5-minute hepatic area massage.Tissue samples were collected 6 hours post-intervention from the model,control,and 6-hour massage groups,and 24 hours post-intervention from the 24-hour massage group.Liver-to-body weight ratios were calculated;The levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),triglycerides(TG),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C),superoxide dismutase(SOD),glutathione(GSH),and malondialdehyde(MDA)in serum and liver homogenates were measured.The liver tissue morphology was observed using hematoxylin-eosin(HE)staining,and fat degeneration in liver cells was examined with Oil Red O staining.Results(1)Compared with the control group,the levels of liver indices,AST,ALT in the model group were increased(P<0.05).The 6-hour massage group exhibited reduced ALT and AST levels compared with the model group(P<0.05),and the 24-hour massage group showed reductions in liver index,ALT,and AST(P<0.05).(2)HE staining:Liver cells in the model group displayed disorganized hepatic cords and damaged lobular structures.Both the 6-hour and 24-hour massage groups showed significant reductions in liver cell damage,with more organized hepatic cords.(3)Compared with the control group,the model group exhibited increased TG and decreased HDL-C(P<0.05).The 6-hour massage group showed decreased TG and increased HDL-C(P<0.05),while the 24-hour massage group had reduced TG an

关 键 词：单次肝区推拿 急性酒精性肝损伤 脂质代谢 护肝 

分 类 号：R244[医药卫生—针灸推拿学]