健脾止动汤对多发性抽动症模型大鼠纹状体组胺受体HRH1、HRH2、HRH3表达的影响  

Effect of Jianpi Zhidong Decoction on the expression of histamine receptors HRH1,HRH2 and HRH3 in the striatum of TS rats

作　　者：史晓伟 王素梅[3] 白雪 赵颖 王倩 崔霞 于文静 SHI Xiaowei;WANG Sumei;BAI Xue;ZHAO Ying;WANG Qian;CUI Xia;YU Wenjing(Beijing University of Chinese Medicine Third Affiliated Hospital,Beijing 100029,China)

机构地区：[1]北京中医药大学第三附属医院推拿科,100029 [2]北京中医药大学第三附属医院儿科,100029 [3]北京中医药大学东方医院儿科

出　　处：《环球中医药》2024年第12期2460-2466,共7页Global Traditional Chinese Medicine

基　　金：国家自然科学基金(82305317);北京市自然科学基金(7182101);中央高校基本科研业务费专项资金(2017-JYB-JS-125);北京中医药大学第三附属医院2022年度院级培育项目(BZYSY-2022-PYQN-06)。

摘　　要：目的探索健脾止动汤对多发性抽动症模型大鼠纹状体组胺受体1(histamine receptor H1,HRH1)、组胺受体2(histamine receptor H1,HRH2)和组胺受体3(histamine receptor H3,HRH3)表达的影响。方法首先将24只SD大鼠按体质量大小随机分为空白组(6只)和造模组(18只),其中造模组应用3,3-亚氨基二丙腈腹腔注射建立多发性抽动模型;造模结束后,再根据刻板运动评分及自主活动总路程结果进行二次分组,将造模组的18只模型大鼠随机分为模型组、中药组和西药组,每组6只,干预6周。干预结束后各组进行刻板运动和自主活动等行为学评价,应用逆转录聚合酶链式反应和免疫蛋白印迹法、免疫组化检测大鼠纹状体HRH1、HRH2和HRH3 mRNA和蛋白表达。结果(1)干预后,与模型组相比,健脾止动汤可显著降低大鼠行为学评分(P<0.01)及自主活动总路程(P<0.05);(2)干预后,与模型组相比,健脾止动汤组及硫必利组纹状体HRH1、HRH2蛋白及mRNA表达水平均无统计学差异(P>0.05);而健脾止动汤可明显下调纹状体HRH3的蛋白(免疫蛋白印迹法P<0.05,免疫组化累积光密度值P<0.01)和HRH3 mRNA表达水平(P<0.05)。结论健脾止动汤通过调控大鼠纹状体HRH3表达发挥抗抽动作用。Objective To explore the effect of Jianpi Zhidong Decoction(JPZDD)on the expression of histamine receptors HRH1,HRH2,and HRH3 in the striatum of tourette syndrome(TS)model rats.Methods 24 SD rats were randomly divided into the blank group(6 rats)and the model group,with 18 rats in each group.The model group was injected with IDPN to establish the TS model.According to behavioral scores and the total distance of autonomous activity,they were randomly divided into the model group,the JPZDD group and the western medicine group,with intervention of 6 weeks.After the intervention,behavioral evaluations(stereotyped behavior and autonomous activity)were conducted,and the mRNA and protein expressions of HRH1,HRH2 and HRH3 in the striatum were detected using the methods of RT-PCR,Western blot,and immunohistochemistry.Results(1)After intervention,JPZDD significantly reduced the behavioral scores(P<0.01)and total distance of autonomous activity(P<0.05)when compared with the model group;(2)After intervention,there was no statistically significant difference in the protein and mRNA expressions of HRH1 and HRH2 in the JPZDD and Western medicine groups when compared with the model group;Compared with the model group,JPZDD significantly reduced the protein expression of HRH3(P<0.05 in Western blot and P<0.01 in IOD value)and mRNA expression of HRH3 in the striatum(P<0.05).Conclusion Jianpi Zhidong Decoction exerts anti tic effects by regulating the expression of HRH3 in the striatum of TS rats.

关 键 词：多发性抽动症 大鼠 健脾止动汤 纹状体 组胺受体 

分 类 号：R285.5[医药卫生—中药学]