基于Toll样受体4/核因子-κB通路探讨针刺对原发性高血压大鼠肾脏巨噬细胞极化调节和肾保护作用  

Modulation of macrophage polarization and nephroprotective effects of acupuncture in SHR kidneys explored based on the TLR4/NF-κB pathway

作　　者：张琳[1] 王方明 郭秋蕾 张静 高辛瑜 吴小丽 刘宇[2] 刘清国[1] ZHANG Lin;WANG Fangming;GUO Qiulei;ZHANG Jing;GAO Xinyu;WU Xiaoli;LIU Yu;LIU Qingguo(School of Acupuncture-Moxibustion and Tuina,Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学针灸推拿学院,100029 [2]北京中医药大学东直门医院针灸科 [3]天津中医药大学第一附属医院针灸部

出　　处：《环球中医药》2024年第12期2474-2480,共7页Global Traditional Chinese Medicine

基　　金：国家自然科学基金(81774413)。

摘　　要：目的探讨捻转补泻手法对原发性高血压大鼠(spontaneous hypertension rat,SHR)血压和肾脏中巨噬细胞极化的影响。方法实验选用12只雄性Wistar-Kyoto(WKY)大鼠作为空白对照组,36只雄性SHR随机分为模型组、捻转泻法组、捻转补法组,每组12只。捻转补法组与捻转泻法组分别在双侧太冲穴进行针刺捻转泻法和针刺捻转补法,每天1次,共两周,中间休息1次。空白对照组与模型组进行相同时长与频次的抓捉固定,不做针刺干预。在干预期间使用尾压法测量大鼠血压,两周后收集大鼠血清和肾组织,采用ELISA法观察血清中去甲肾上腺素(norepinephrine,NE)、血管紧张素Ⅱ(AngiotensinⅡ,AngⅡ)含量和肾脏中白细胞介素(interleukin,IL)-1β、IL-10含量。Western blot法观察肾脏中Toll样受体4(Toll-like receptor 4,TLR4)、核转录因子-κB(nuclear factor-κB,NF-κB)、分化簇(cluster of differentiation,CD)86、CD206蛋白的表达情况。用苏木素—伊红(hemotoxylin-eosin,HE)染色观察肾组织形态。结果(1)与空白对照组相比,模型组大鼠血压显著升高(P<0.01),血清NE、AngⅡ浓度显著上升(P<0.01),肾脏中TLR4、NF-κB、CD86、IL-1β含量显著上升(P<0.01),CD206、IL-10含量显著下降(P<0.01)。HE染色结果显示,SHR出现肾小管与肾小球形态不规则,存在炎性细胞浸润。(2)与模型组相比,捻转泻法组和捻转补法组血压显著降低(P<0.01),血清NE、AngⅡ浓度显著降低(P<0.01),肾中TLR4、NF-κB、CD86表达显著下降(P<0.01),IL-1β浓度降低(P<0.05,P<0.01),CD206、IL-10含量显著上升(P<0.01),肾小管与肾小球形态正常,存在少量炎性细胞浸润。(3)与捻转泻法组相比,捻转补法组血压较高(P<0.05),血清中NE、AngⅡ浓度和肾脏中TLR4、NF-κB、CD86、CD206、IL-1β、IL-10均存在差异,且差异具有统计学意义(P<0.05,P<0.01)。结论针刺太冲穴并行捻转补泻手法通过降低交感神经兴奋性降低AngⅡ含量,抑制TLR4/NF-κB通路调�Objective To observe the effects of acupuncture manipulation on blood pressure and renal inflammatory environment in spontaneously hypertensive rats(SHR),and to elucidate the mechanism of antihypertensive and nephroprotective effects of the manipulation.Methods 36 male SHRs were randomly divided into 3 groups including the model group(SHR group),the twirling reducing manipulation group(TRD group)and the twirling reinforcing manipulation group(TRF group).In addition,12 WKY rats were used as the control group(WKY group).The TRD and the TRF groups were subjected to twirling reducing manipulation and twirling reinforcing manipulation in LR3 respectively,once a day for two weeks,with one day of rest in between the two weeks.During the intervention period,we measured the blood pressure of the rats using the tail pressure method.After two weeks,the serum and kidney tissues of rats in each group were collected,and the concentrations of norepinephrine(NE)and AngiotensinⅡ(AngⅡ)in serum and the contents of interleukin(IL)-1β and IL-10 in kidneys were detected by Elisa method;the expressions of Toll-like receptor 4(TLR4),nuclear factor-κB(NF-κB),cluster of differentiation(CD)86 and CD206 proteins in kidneys were detected by Western blot method;and morphological changes of kidneys were observed by HE staining.Results(1)Compared with the WKY group,the blood pressure of rats in the SHR group was significantly increased(P<0.01),serum NE and AngⅡ concentrations were significantly increased(P<0.01),and the content of TLR4,NF-κB,CD86,IL-1β in kidney was significantly increased(P<0.01)and the content of CD206 and IL-10 was significantly decreased(P<0.01).The results of HE staining showed that SHRs appeared irregular renal tubular and glomerular morphology,with the presence of inflammatory cell infiltration.(2)Compared with SHR group,blood pressure was significantly decreased in the TRD group and the TRF group(P<0.01),serum NE and AngⅡ concentrations were significantly decreased(P<0.01).The expression of TLR4,NF-κB,CD86

关 键 词：太冲 针刺 捻转补泻手法 巨噬细胞极化 Toll样受体4/核因子-κB通路 自发性高血压 肾保护 交感神经系统 

分 类 号：R245[医药卫生—针灸推拿学]