Uniting the Role of Entomopathogenic Fungi against Rhizoctonia solani JG Kühn, the Causal Agent of Cucumber Damping-Off and Root Rot Diseases  

作　　者：Abdelhak Rhouma Lobna Hajji-Hedfi Nahla Alsayd Bouqellah Pravin Babasaheb Khaire Samar Dali Omaima Bargougui Amira Khlif Laith Khalil Tawfeeq Al-Ani 

机构地区：[1]Research Laboratory of Agricultural Production Systems and Sustainable Development(LR03AGR02),Carthage University,Regional Centre of Agricultural Research of Sidi Bouzid,CRRA,Sidi Bouzid,9100,Tunisia [2]Science College,Biology Department,Taibah University,Almadina,42317,Saudi Arabia [3]Department of Plant Pathology and Microbiology,Mahatma Phule Krishi Vidyapeeth,Rahuri,413722,Maharashtra,India [4]School of Biology Science,Universiti Sains Malaysia,Minden,11800,Malaysia

出　　处：《Phyton-International Journal of Experimental Botany》2024年第11期2857-2881,共25页国际实验植物学杂志（英文）

摘　　要：Beauveria bassiana and Metarhizium spp.are entomopathogenic fungi with potential applications beyond insect pest control,including plant disease suppression,plant growth promotion,and rhizosphere colonization.This study investigated the plant growth-promoting characteristics and extracellular enzyme activities of Metarhizium spp.and B.bassiana in relation to phytopathogen interactions and plant growth.Additionally,the efficacy of these fungi in mitigating damping-off and root rot caused by Rhizoctonia solani on cucumber plants was evalu-ated in vitro and in vivo.Results indicate that B.bassiana and M.anisopliae produce indole-3-acetic acid,hydro-cyanic acid,and hydrolytic enzymes.Seed treatment with these fungi significantly reduced disease severity(3.85%–1.86%,respectively)and enhanced germination parameters[germination percentage(85.33%–86%,respectively),germination index(10.67–12.29,respectively),seedling length vigor index(86.41–109.44,respec-tively),and seedling weight vigor index(30.24–37.57,respectively)]compared to the control positive.Both fungi demonstrated high inhibition rates of R.solani mycelial growth(93.90%–90.46%,respectively).Greenhouse trials revealed that preventive treatments using B.bassiana and M.anisopliae increased catalase(104.40–105.52 units/mg protein/min,respectively),(4.58–5.77 units/mg protein/min,respectively),superoxide dismutase(40.65–41.74 units/mg protein/min,respectively),and polyphenol oxidase(0.539–0.559 units/mg protein/min,respectively)activities,as well as total phenolic(2.60–2.65 mg/g,respectively)and total sugar content(2.23–2.16 mg/g,respectively)in cucumber plants.Consequently,disease severity(9.51–6.99%,respectively)was reduced,and plant height(93.76–98.76 cm,respectively)increased compared to the positive control.Thesefind-ings suggest that B.bassiana and M.anisopliae can enhance plant growth,stimulate plant defense mechanisms,and effectively control damping-off and root rot diseases,making them promising candidates for biological con-

关 键 词：Metarhizium spp. Beauveria bassiana BIOCONTROL plant growth promotion Cucumis sativus 

分 类 号：Q94[生物学—植物学]