Seed Priming Improves Chilling Stress Tolerance in Rice (Oryza sativa L.) Seedlings  

作　　者：Md.Tahjib-Ul-Arif Md Asaduzzaman Bir Jahangir Shirazy Md.Shihab Uddine Khan A.M.Sajedur Rahman Yoshiyuki Murata Sozan Abdel Hamed Arafat Abdel Hamed Abdel Latef 

机构地区：[1]Department of Biochemistry and Molecular Biology,Bangladesh Agricultural University,Mymensingh,2202,Bangladesh [2]Graduate School of Environmental,Life,Natural Science and Technology,Okayama University,Okayama,700-8558,Japan [3]Atopy(Allergy)Research Center,Graduate School of Medicine,Juntendo University,Tokyo,113-8421,Japan [4]Rice Farming Systems Division,Bangladesh Rice Research Institute,Gazipur,1701,Bangladesh [5]Agricultural Research Station,Bangladesh Agricultural Research Institute,Satkhira,9400,Bangladesh [6]Breeding Division,Bangladesh Sugarcrop Research Institute,Pabna,6620,Bangladesh [7]Department of Horticulture,Faculty of Agriculture,South Valley University,Qena,83523,Egypt [8]Botany and Microbiology Department,Faculty of Science,South Valley University,Qena,83523,Egypt [9]Molecular Biology and Biotechnology Center,South Valley University,Qena,83523,Egypt

出　　处：《Phyton-International Journal of Experimental Botany》2024年第11期3013-3027,共15页国际实验植物学杂志（英文）

摘　　要：Chilling is one of the major abiotic stresses for plants,especially for rice cultivation.Many essential metabolic processes for growth and development are temperature-dependent.In that case,reducing the negative effects of cold stress using exogenous chemicals is a possible option.Therefore,the current study examined the effects of pre-sowing seed treatment with different chemicals,viz.hydrogen peroxide(H_(2)O_(2)),salicylic acid(SA),calcium chloride(CaCl_(2)),thiourea(TU),and citric acid(CA)on the germination of rice seeds(cv.BRRI dhan28)under chilling environments.Rice seeds were soaked in distilled water(control),10 mM CA,2 mM SA,10 mM CaCl_(2),10 mM H_(2)O_(2),and 10 mM TU solutions for 24 h.After that,seeds were exposed to chilling stress by incubating at 4±1℃ for 8 h,followed by at 25±2℃ for 16 h for 7 days.Exposure to chilling stress significantly reduced thefinal germination percent(13.6%),germination rate index(36.0%),coefficient of the velocity(25.0%),shoot fresh weight(44.4%),and root fresh weight(60.5%).Moreover,chilling induced oxidative damage and reduced the activity of antioxidant enzymes(catalase and ascorbate peroxidase).In contrast,treatments with H_(2)O_(2),SA,CaCl_(2),TU,and CA considerably enhanced germination indices and seedling growth compared to chilling stress condi-tions.The study showed that priming with H_(2)O_(2),SA,CaCl_(2),TU,and CA significantly boosted antioxidant enzyme activities and reduced MDA and H_(2)O_(2) contents in chilling-stressed rice plants,indicating less oxidative stress and improved tolerance.Principal component analysis showed that among these priming agents,H_(2)O_(2),SA,and CA are most effective in chilling stress mitigation.Therefore,using seed-treating chemicals to combat the effect of chilling stress can help rice seedlings grow better in the winter season.

关 键 词：Chilling stress antioxidant enzymes germination indices cold injury seed priming oxidative stress 

分 类 号：S511[农业科学—作物学]