EMS-Mediated Mutagenesis in Marigold Seeds and Its Effects on Seedling Growth and Physiology  

作　　者：Chao Meng Ikram Ullah Wenjin Wu Yiping Zhang Ruixue Shi Shaodan Luo Cuixia Luo Satyabrata Nanda Mahmoud FSeleiman Yalian Jiang Wangqi Huang 

机构地区：[1]Institute of Flower,Yunnan Academy of Agricultural Sciences,Yunnan Key Laboratory for Flower Breeding,National Engineering Research Center for Ornamental Horticulture,Yunnan Provincial Key Laboratory of Flower Genetic Improvement,Kunming,650205,China [2]College of Horticulture,Northwest A&F University,Yangling,712100,China [3]West Yunnan University,Lincang,67700,China [4]School of Biotechnology,Centurion University of Technology and Management,Bhubaneswar,752050,India [5]Plant Production Department,College of Food and Agriculture Sciences,King Saud University,Riyadh,11451,Saudi Arabia

出　　处：《Phyton-International Journal of Experimental Botany》2024年第11期3029-3038,共10页国际实验植物学杂志（英文）

基　　金：This work was supported by Yunnan Provincial Department of Science and Technology Key R&D Plan(202303AM140018,202303AK140029,202303AK140028);Yunnan Flower Breeding Key Experiment Open Foundation(FKL-202203);Yunnan Provincial Department of Science and Technology Science and Technology Project Agriculture Joint Foundation(202301BD070001-208);Yunnan Provincial Expert Basic Research Workstation Foundation;We also acknowledge the financial support from the Researchers Supporting Project(RSPD2025R751),King Saud University,Riyadh,Saudi Arabia.

摘　　要：Marigolds(Tagetes spp.)are popular horticultural plants worldwide.The current study aimed to investigate the optimal mutagenic conditions for marigold seeds using EMS(ethyl methanesulfonate)mutagenesis.Different con-centrations and treatment times of EMS were applied to investigate their effects on the marigold seed germination rate,growth traits,antioxidant enzyme activities(i.e.,SOD and POD),and malondialdehyde(MDA)contents.Results indicated that with increasing the EMS treatment duration and concentration,the seed germination rate and growth treatments were reduced,accompanied by elevated MDA content.In addition,SOD and POD activ-ities initially correlated positively with the growth tratis at the lowest concentrations and shortest durations of EMS,but such relationship diminished beyond certain thresholds.The comprehensive analysis identified the opti-mal mutagenic conditions as 1%EMS treatment for 12 h,achieving a semi-lethal dose and enhancing stress-resis-tant components in seedlings.Thesefindings are pivotal for advancing genetic enhancement and germplasm innovation in marigolds.

关 键 词：Marigolds seed germination EMS mutagenesis genetic enhancement 

分 类 号：Q94[生物学—植物学]