Novel Insights into the Conservation Physiology and Ex situ Conservation of the Threatened and Rare Semi-Aquatic Moss Drepanocladus lycopodioides (Amblystegiaceae)  

作　　者：Bojana Z.Jadranin Marija V.Cosic Djordje P.Bozovic Milorad M.Vujicic Beata Papp Aneta D.Sabovljevic Marko S.Sabovljevic 

机构地区：[1]Institute of Botany and Botanical Garden“Jevremovac”,Faculty of Biology,University of Belgrade,Belgrade,RS-11000,Serbia [2]Center of Plant Biotechnology and Conservation(CPBC),Belgrade,RS-11000,Serbia [3]Department of Botany,Hungarian Natural History Museum,Budapest,HU-1476,Hungary [4]Department of Plant Biology,Institute of Biology and Ecology,Faculty of Science,Pavol Jozef Safarik University in Kosice,Kosice,SK-04001,Slovakia

出　　处：《Phyton-International Journal of Experimental Botany》2024年第11期3039-3054,共16页国际实验植物学杂志（英文）

基　　金：The project is supported by the Serbian Ministry of Science,Technological Development and Innovations,contract nos.451-03-65/2024-03/200178 and 451-03-66/2024-03/200178.

摘　　要：The rare and threatened semi-aquatic moss Drepanocladus lycopodioides(Amblystegiaceae)was the subject of growth optimization under ex situ axenic laboratory conditions.The positioning of the plantlets on media,media types as well as selected growth regulators and sugars were parameters tested in optimizing growth promotion of this species in captivity.Out of the tested media types,the KNOP medium and the upright positioning of the explants were the best for propagation and biomass production of D.lycopodioides.The addition of sugars had no significant effect on this moss development axenically,while exogenously applied Benzylaminopurine(BAP)at a concentration of 3µM induced the development of archegonia on the sterile gametophores and can be used as a marker for confirmation of the female sex,by inducing archegonia on the sterile female plants or clones.This study aimed to contribute to the conservation of this threatened moss species,by massive propa-gation for reintroduction and population straitening in natural and semi-natural habitats,in scenarios of rapid climate change and water shortage.Laboratory experiments were carried out to study the biological features of the species itself,as due to its rarity empirical data are lacking.This research contributed to the conservation and multiplication of D.lycopodioides and suggested an elegant method to prevent the extinction of this species from its natural habitats.This enables experimental investigation in this species and directs a plan of action for species survival both in captivity(ex situ)and in nature(in situ).

关 键 词：BRYOPHYTES growth development protection biomass 

分 类 号：Q94[生物学—植物学]