Bioactive Compounds Recovery from Larrea tridentata by Green Ultrasound- Assisted Extraction  

作　　者：Muyideen Olaitan Bamidele Jose Sandoval-Cortes Maria Liliana Flores-Lopez Olga Berenice Alvarez Perez Monica Lizeth Chavez Gonzalez Cristobal Noe Aguilar 

机构地区：[1]Bioprocesses and Bioproducts Research Group,Food Research Department,School of Chemistry,Universidad Autonoma de Coahuila,Saltillo,25070,Mexico [2]Centro de Investigacion e Innovacion Cientifica y Tecnologica,Universidad Autonoma de Coahuila,Saltillo,25070,Mexico

出　　处：《Phyton-International Journal of Experimental Botany》2024年第11期3091-3107,共17页国际实验植物学杂志（英文）

基　　金：This work was supported by the appreciated National Council of Humanities,Sciences and Technologies(CONAHCYT,Mexico),through the PhD fellowship grant(No.1245491)awarded to Muyideen Olaitan Bamidele.

摘　　要：Postharvest losses remain a significant challenge,particularly in developing countries that have inadequate infra-structure.Medicinal plants offer an eco-friendly and sustainable solution for managing diseases and pests in agri-cultural systems.These plants are rich in bioactive compounds,such as alkaloids,flavonoids,terpenoids,phenolics,and essential oils,many of which have proven antimicrobial,antifungal,insecticidal,and antioxidant properties.These characteristics make medicinal plants viable candidates for postharvest disease and pest control.Among these,Larrea tridentata(creosote bush)is particularly notable for its bioactive compounds with strong antifungal properties.Their potential applications include agriculture,food preservation,and medicine.This study aimed to evaluate how different solvent mixtures affect the efficiency of ultrasound-assisted extraction,total polyphenol content,antioxidant capacity,and antifungal activity of L.tridentata leaves and stems.Thefindings revealed that the 60%ethanol ultrasound-assisted extract of L.tridentata leaves(ULL 60%)contained the highest concentration of bioactive compounds,including hydrolysable tannins(690.2 mg GAE/100 g)and condensed tan-nins(329.9 mg CE/100 g).All extracts demonstrated notable antioxidant activity in ABTS,DPPH,and FRAP assays,with ethanol-based extracts showing greater antioxidant potential than their aqueous counterparts.In terms of antifungal efficacy,100%ethanol leaf extract exhibited the strongest inhibition against Fusarium oxyspor-um(60.03%),whereas 50%ethanol extract effectively inhibited Alternaria alternata(53.61%).Six major polyphe-nolic compounds were identified using reverse-phase high-performance liquid chromatography coupled with electrospray ionization mass spectrometry(RP-HPLC-ESI-MS).These include quercetin,luteolin,3,4-dihydrox-yphenylethanol,elenolic acid,nordihydroguaiaretic acid(NDGA),and kaempferide.These compounds are known to have antibacterial,antifungal and antioxidant properties.Thesefindings underscore the po

关 键 词：PHYTOPATHOGENS bioactive compounds desert plants Fusarium oxysporum Alternaria alternata 

分 类 号：Q94[生物学—植物学]