羟苯磺酸钙对基于Trinder反应原理的血清ADA、 Crea、UA、TG、TC、GSP水平的影响  

Effects of Calcium Isobenesulfonate on Serum ADA,Crea,UA,TG,TC and GSP Levels Based on Trinder Reaction Principle

作　　者：周茜宁 高静[1] 张旭[1] 冯杰[1] 高艳红[1] ZHOU Xining;GAO Jing;ZHANG Xu;FENG Jie;GAO Yanhong(Clinical Laboratory,the First Medical Center of the PLA General Hospital,Beijing 100853,China)

机构地区：[1]解放军总医院第一医学中心检验科,北京100853

出　　处：《标记免疫分析与临床》2024年第11期2051-2055,共5页Labeled Immunoassays and Clinical Medicine

基　　金：国家重点研发计划课题(编号:2017YFF0205401)。

摘　　要：目的研究羟苯磺酸钙对血清生化检测结果的影响。方法10份血清分别加入10mg/L羟苯磺酸钙的血清为实验样品,测定各生化项目数值,计算偏倚,筛选出平均偏倚大于1/2总允许误差(TE)的项目。配置50、40、30、20、10、5mg/L羟苯磺酸钙干扰的血清,检测平均偏倚大于1/2 TE的项目结果,计算偏倚。结果羟苯磺酸钙浓度为10mg/L时,ADA、Crea、UA、TG、TC、GSP 6个项目的偏倚大于1/2 TE。随着羟苯磺酸钙浓度的增高,Crea、UA、TG、TC、GSP、ADA与真实水平的平均偏倚呈负向增高状态。此6项测试在羟苯磺酸钙浓度为5mg/L时,平均偏倚最低,分别为-10.47%、-6.44%、-7.16%、-5.53%、-10.95%、-18.87%;在羟苯磺酸钙浓度为50mg/L时,平均偏倚最高,分别为-42.99%、-22.23%、-27.84%、-9.83%、-36.08%、-57.32%。结论羟苯磺酸钙对基于Trinder反应原理的血清生化检测有影响,随着样本中羟苯磺酸钙浓度的增大,相对偏倚负向增大。Objective To study the effect of calcium dobesilate on serum biochemical test results.Methods 10 serum supplemented with 10mg/L calcium dobesilate were selected as experimental samples.The values of each biochemical item were determined,while the calculation bias was calculated,and items whose average bias was greater than 1/2 of the total allowable error were selected.Serum samples with 50mg/L,40mg/L,30mg/L,20mg/L,10mg/L,and 5mg/L calcium dobesilate interference were configured to detect the results of items with an average bias greater than 1/2TE and then calculated for the bias.Results When the concentration of calcium dobesilate was 10mg/L,the bias of ADA,Crea,UA,TG,TC and GSP were greater than 1/2TE.Calcium dobesilate affected those biochemical tests based on Trinder chromogenic reaction.With the increasing concentration of calcium dobesilate in samples,increasing interferences and biases were observed.Negative correlations were also found between serum results and interference concentrations.The mean bias between Crea,UA,TG,TC,GSP,ADA and the true level increased negatively with the increase of calcium dobesilate concentration.The average bias of the six tests was lowest when the concentration of calcium dobesilate was 5mg/L,which were-10.47%,-6.44%,-7.16%,-5.53%,-10.95%and-18.87%,respectively.When the concentration of calcium dobesilate was 50mg/L,the average bias was the highest,which were-42.99%,-22.23%,-27.84%,-9.83%,-36.08%and-57.32%,respectively.Conclusion Calcium dobesilate can affect serum biochemical detection results based on Trinder reaction principle,and the relative bias increases negatively with the increase of the concentration of calcium dobesilate in testing samples.

关 键 词：羟苯磺酸钙 TRINDER反应 偏倚 干扰 

分 类 号：R392-33[医药卫生—免疫学]