丝棉木组培快繁体系的优化  

Optimization of tissue culture and rapid propagation system of Euonymus maackii Rupr.

作　　者：师群航 刘艳军 易元慧 Shi Qunhang;Liu Yanjun;Yi Yuanhui(College of Horticulture and Landscape,Tianjin Agricultural University,Tianjin 300392,China)

机构地区：[1]天津农学院园艺园林学院,天津300392

出　　处：《天津农学院学报》2024年第6期39-43,共5页Journal of Tianjin Agricultural University

摘　　要：以一年生丝棉木带腋芽休眠枝条的茎段为材料,研究不同消毒时间、不同基本培养基、外源激素选择以及激素间配比对丝棉木离体培养的影响,优化现有的丝棉木组培快繁体系。试验结果表明丝棉木带腋芽茎段用70%酒精消毒30 s,再用2%次氯酸钠消毒12 min,其污染率最低,成活率最高;使用MS培养基并添加5.0 mg/L GA_3+125 mg/L羧苄青霉素钠作为初代培养基,腋芽萌发率最高,生长状况最好;适宜丝棉木腋芽继代增殖培养基为1/2MS+0.5 mg/L IAA+1.0 mg/L BA,培养基中均添加琼脂7.0 g/L,30 g/L蔗糖,30 d后有效增殖系数最高,为4.2;生根培养条件最佳为1/2MS+0.5mg/LNAA;炼苗移栽阶段使用草炭∶蛭石∶珍珠岩=1∶1∶1(v/v)的基质,移栽30 d后有87株丝棉木长势良好且根系完整、健壮,其移栽成活率为96.67%。Using the stem segments with dormant shoots of axillary buds of annual silk cotton wood as materials,the effects of different disinfection times,different basic media,selection of exogenous hormones and ratio between hormones on the culture in vitro of silk cotton wood were studied,and the existing tissue culture rapid propagation system of silk cotton wood was optimized.The test results showed that the pollution rate was the lowest and the survival rate was the highest when the axillary bud stem segment of silk cotton wood was disinfected with 70%alcohol for 30s,and then disinfected with 2%sodium hypochlorite for 12min.Using MS medium and adding 5.0 mg/L GA3+125 mg/L carboxybenzicillin sodium as the primary medium,axillary bud germination rate was the highest and growth status was the best.The suitable subproliferation medium for axillary buds of silk cotton wood was 1/2MS+0.5 mg/L IAA+1.0 mg/L BA,and the effective proliferation coefficient was the highest after 30 days,which was 4.2,and the optimal rooting culture conditions were 1/2MS+0.5 mg/L NAA.The substrate of grass peat∶vermiculite∶perlite=1∶1∶1(v/v)was used in the transplanting stage,and 87 silk cotton trees grew well and had intact root systems after 30 days of transplanting,and the survival rate of transplanting was 96.67%.

关 键 词：丝棉木 组织培养 带腋芽茎段 植物生长调节剂 

分 类 号：S567.19[农业科学—中草药栽培]