抗咪唑啉酮类除草剂广亲和水稻恢复系的诱变筛选鉴定  

Mutation screening and identification of wide-compatibility restorer rice line resistant to imidazolinone herbicide

作　　者：董俊杰 张馨月 富昊伟[1] 李友发[1] DONG Junjie;ZHANG Xinyue;FU Haowei;LI Youfa(Jiaxing Academy of Agricultural Sciences,Jiaxing 314016,Zhejiang)

机构地区：[1]嘉兴市农业科学研究院,浙江嘉兴314016

出　　处：《浙江农业科学》2024年第12期2826-2829,共4页Journal of Zhejiang Agricultural Sciences

基　　金：嘉兴市科研项目(2022AZ10003);嘉兴市科技特派员专项(K2022C003);浙江省市级农科院联盟区域示范性项目(2023SJLM09)。

摘　　要：培育抗除草剂的水稻品种是防治稻田杂草最经济有效的途径。咪唑啉酮类除草剂以乙酰乳酸合成酶(acetolactate synthase,ALS)为靶标,能防除绝大多数种类的稻田常见杂草。本研究通过350 Gy^(60)Co-γ射线和EMS诱变剂诱变广亲和性恢复系DR610,筛选到12份抗咪唑啉酮类除草剂水稻材料。通过与三系粳稻不育系嘉14A、长粳1A、浙粳7A分别测配,筛选到3份广亲和恢复系。进一步的序列分析表明,ALS基因的第1880位由G碱基突变为A碱基,导致第627位氨基酸由丝氨酸突变为天冬酰胺,赋予了3份广亲和恢复系的除草剂抗性。本研究首次通过双重诱变来筛选抗除草剂的广亲和恢复系,为抗除草剂籼粳杂交水稻新品种的培育奠定了基础。Breeding for rice varieties with resistance to imidazolinone herbicides is one of the most economical and effective strategies to control weeds in rice fields.Imidazolinone herbicides target acetyllactate synthase and can control the vast majority of common weeds in rice fields.In this study,we screened 12 rice materials resistant to imidazolinone herbicides by inducing the wide-compatibility restorer line DR610 with 350 Gy^(60)Co-γradiation and EMS mutagens.Through pairing with the three japonica male sterile lines J14A,Changgeng 1A,and Zhegeng 7A,three wide-compatibility restoring lines were ultimately screened.Furthermore,sequence analysis showed that G/A mutation at position 1880 of ALS gene,which led to 627th amino acid alteration from serine to asparagine,conferring herbicide resistance to three wide-compatibility restoring lines.This study was the first to screen herbicide-resistant wide-compatibility restorer lines by double mutagenesis with a practical potential in breeding for new herbicide-resistant indica-japonica hybrid rice varieties.

关 键 词：水稻 咪唑啉酮 除草剂 广亲和 诱变 

分 类 号：S511[农业科学—作物学]