南荻纤维素分批补料同步糖化发酵产乙醇工艺优化及其结构表征  

Optimization of ethanol production process and structural characterization of Miscanthus lutarioriparius cellulose by fed-batch synchronous saccharification and fermentation

作　　者：齐慧[1] 彭志利 陈亮[1] 刘安 邓明[1] 王克勤[1] 苏小军 武小芬[1] Qi Hui;Peng Zhili;Chen Liang;Liu An;Deng Ming;Wang Keqin;Su Xiaojun;Wu Xiaofen(Hunan Institute of Nuclear Agricultural Science and Space Breeding,Hunan Academy of Agricultural Sciences,Changsha 410125,China;Food Science and Technology College,Hunan Agricultural University,Changsha 410125,China)

机构地区：[1]湖南省农业科学院核农学与航天育种研究所,湖南长沙410125 [2]湖南农业大学食品科学技术学院,湖南长沙410125

出　　处：《可再生能源》2024年第12期1578-1586,共9页Renewable Energy Resources

基　　金：国家自然科学基金青年基金项目(12005058);湖南省自然科学基金青年基金项目(2020JJ5286)。

摘　　要：文章以南荻纤维素为原料,采用分批补料同步糖化发酵工艺生产生物乙醇,分析了底物初始质量分数、底物最终质量分数、补料次数对乙醇产量的影响,并在此基础上采用正交试验进行工艺优化,获得最佳工艺参数:底物初始质量分数为17.5%,分批补料2次至底物最终质量分数为32.5%。在此条件下,乙醇含量最高可达70.15 g/L,较未补料同步糖化发酵过程获得的最大乙醇含量提高23.36%。在分批补料同步糖化发酵最佳工艺的基础上,做了物料平衡分析,并采用扫描电镜、傅里叶变换红外光谱、X-衍射对发酵前、后的南荻纤维素结构进行表征,结果显示,酶解糖化及发酵过程会改变南荻纤维素的内部结构,使氢键或共价键断裂,底物结晶度提高。In this paper,Miscanthus lutarioriparius(ML)cellulose was used as the raw material to produce bioethanol by a fed-batch synchronous saccharification and fermentation process.The effects of initial substrate concentration,final substrate concentration,and feeding times on ethanol yield were analyzed.The orthogonal experiments were used to optimize the process,and the optimal process parameters were obtained as follows:Initial substrate concentration was 17.5%,and the batch feeding was conducted twice until the final substrate concentration was 32.5%.Under these conditions,the highest ethanol content could reach to 70.15 g/L,which increased by 23.36%compared to the synchronous saccharification and fermentation process without fed-batch.The material balance analysis was conducted on the basis of the optimal process.The structure of ML cellulose before and after fermentation was characterized by scanning electron microscopy,fourier transform infrared spectroscopy,and X-ray diffraction.The results showed that the enzymatic saccharification and fermentation process would change the internal structure of ML cellulose,break the hydrogen bond or covalent bond,and increase the crystallinity of the substrate.

关 键 词：南荻纤维素 分批补料 同步糖化发酵产乙醇 工艺优化 物料平衡 结构表征 

分 类 号：TK6[动力工程及工程热物理—生物能]