人类趋化素样因子超家族2在自身免疫性睾丸炎模型下参与调控小鼠睾丸中精子生成  

CMTM2 is involved in spermatogenesis regulation in the testis of the mouse model of experimental autoimmune orchitis

作　　者：徐俊泰 马红斌 王德克 田振方 XU Jun-tai;MA Hong-bin;WANG De-ke;TIAN Zhen-fang(Department of Urology,Women and Children's Hospital,Qingdao University,Qingdao,Shandong 266000,China;Qingdao University,Qingdao,Shandong 266071,China;The Fifth People's Hospital of Qingdao West Coast New Area,Qingdao,Shandong 266425,China;Department of Urology,Juye County People's Hospital Heze,Shandong 274900,China)

机构地区：[1]青岛大学附属妇女儿童医院泌尿外科,山东青岛266000 [2]青岛大学,山东青岛266071 [3]青岛西海岸新区第五人民医院,山东青岛266425 [4]巨野县人民医院泌尿外科,山东菏泽274900

出　　处：《中华男科学杂志》2024年第12期1110-1116,共7页National Journal of Andrology

摘　　要：目的:构建实验性自身免疫性睾丸炎(EAO)小鼠模型,探究人类趋化素样因子超家族2(CMTM2)在调控小鼠睾丸精子生成过程中的作用。方法:在CMTM2基因敲除小鼠及野生型小鼠中采用EAO模型,评价CMTM2基因敲除小鼠的免疫学生殖表型,统计分析基因敲除组的精子生成数量、形态及活性,分析睾丸组织切片、睾丸间质中巨噬细胞及淋巴细胞浸润程度;利用RT-PCR及Western、Northern印迹技术检测CMTM2基因敲除型纯合子、杂合子及野生型小鼠的CMTM2表达水平。结果:CMTM2基因敲除纯合子小鼠与野生型小鼠成年后,两组睾丸长轴及精子生成数量比较,差异具有统计学意义(P<0.05);CMTM2敲除小鼠EAO模型睾丸中巨噬细胞及淋巴细胞总数较野生型小鼠EAO模型上升,精子活性及正常形态比例下降。结论:CMTM2基因作为生精调控基因,在成年雄性小鼠中高度表达,在维持精子发生的过程中呈重要作用,且在慢性炎症状态下CMTM2基因表达产物减少导致精子发生减弱。Objective:To explore the role of the CMTM2 gene in regulating testicular spermatogenesis in the mouse model of experimental autoimmune orchitis(EAO).Methods:We constructed an EAO model in CMTM2 knockout and wild-type(WT)mice,studied the immunological reproductive phenotype and examined the number,morphology and activity of the sperm generated in the CMTM2 knockout mice.We assessed the infiltration of macrophages and lymphocytes in the testis tissue sections and Leydig cells,and determined the expression levels of CMTM2 in the homozygous knockout(KO),heterozygous and WT mice by RT-PCR,Western blot and Northern blot.Results:Statistically significant differences were observed in the long testicular axis and the number of sperm generated between the KO and WT mice after reaching adulthood(P<0.05).The total numbers of macrophages and lymphocytes were markedly increased,while sperm motility and the percentage of morphologically normal sperm remarkably decreased in the testis of the KO mouse model compared with those in the WT mouse model of EAO.Conclusion:The CMTM2 gene,as a regulator of spermatogenesis,is highly expressed in adult male mice and plays an important role in the maintenance of spermatogenesis.Moreover,decreased expression products of the CMTM2 gene may weaken spermatogenesis under chronic inflammation conditions.

关 键 词：人类趋化素样因子超家族2 精子发生 男性不育 自身免疫性睾丸炎 小鼠 

分 类 号：R698.2[医药卫生—泌尿科学] Q95[医药卫生—外科学]