高雌激素暴露对妊娠期高血压孕鼠胎盘损伤和免疫功能的调节作用研究  

Study on the Regulatory Effect of High Estrogen Exposure on Placental Damage and Immune Function in Pregnant Rats with Gestational Hypertension

作　　者：时玲玲 吴桂杰 王艳 李彦玲 刘春双 SHI Ling-ling;WU Gui-jie;WANG Yan;LI Yan-ling;LIU Chun-shuang(Department of Gynaecology and Obstetrics,Cangzhou People's Hospital of Hebei Province,Cangzhou,Hebei,061000,China)

机构地区：[1]河北省沧州市人民医院妇产科,河北沧州061000

出　　处：《现代生物医学进展》2024年第23期4417-4420,4448,共5页Progress in Modern Biomedicine

基　　金：2022年度河北省医学科学研究课题计划项目(20220316)。

摘　　要：目的:探讨高雌激素暴露对妊娠期高血压(GH)孕鼠胎盘损伤和免疫功能的调节作用。方法:构建GH大鼠模型,将大鼠随机分为对照组(Control组)、GH组、雌激素低剂量组(L-estrogen组)、雌激素中剂量组(M-estrogen组)、雌激素高剂量组(H-estrogen组)。检测孕鼠收缩压、舒张压、24 h尿蛋白、促卵泡激素(FSH)、雌二醇(E2)、促黄体生成素(LH)、肿瘤坏死因子α(TNF-α)、γ干扰素(IFN-γ)、白细胞介素(IL)-6、IL-17含量、辅助性T细胞(Th)17/调节性T细胞(Treg)细胞比例;观察孕鼠胎盘组织病理变化和胎盘滋养细胞超微结构。结果:GH组孕鼠绒毛数量减少,滋养层细胞核固缩,收缩压、舒张压、24 h尿蛋白、Th17细胞比例、Th17/Treg比值、IFN-γ、TNF-α、IL-6、IL-17含量显著增加,FSH、E2、LH、Treg细胞比例显著降低(P<0.05);与GH组比较,L-estrogen组、M-estrogen组、H-estrogen组孕鼠胎盘组织损伤、滋养细胞核固缩减轻,收缩压、舒张压、24 h尿蛋白、Th17细胞比例、Th17/Treg比值、IFN-γ、TNF-α、IL-6、IL-17含量显著降低,FSH、E2、LH、Treg细胞比例显著增加,且呈剂量依赖性(P<0.05)。结论:雌激素暴露可降低GH大鼠血压,改善GH孕鼠胎盘损伤和免疫功能,以高剂量雌激素的干预效果更佳。Objective:To investigate the regulatory effect of high estrogen exposure on placental damage and immune function in pregnant rats with gestational hypertension(GH).Methods:GH rat model was constructed,and rats were randomly divided into control group(Control group),GH group,low-dose estrogen group(L-estrogen group),medium-dose estrogen group(M-estrogen group)and high-dose estrogen group(H-estrogen group).The systolic pressure,diastolic pressure,24 h urine protein,follicle stimulating hormone(FSH),estradiol(E2)and luteinizing hormone(LH),tumor necrosis factor-α(TNF-α),interferon-γ(IFN-γ),interleukin(IL)-6,IL-17 contents,and the proportion of helper T cells(Th)17/regulatory T cells(Treg)of pregnant rats were detected.The pathological changes of placental tissue and the ultrastructure of placental trophoblast cells in pregnant rats were observed.Results:The number of villi in GH group was decreased,the nucleus of trophoblast cells was pyknosis,systolic blood pressure,diastolic blood pressure,24 h urine protein,Th17 cell ratio,Th17/Treg ratio,IFN-γ,TNF-α,IL-6 and IL-17 were significantly increased,and the proportion of FSH,E2,LH and Treg cells was significantly decreased(P<0.05).Compared with GH group,the placental tissue damage and nuclear pyknosis of trophoblast cells were reduced in L-estrogen group,M-estrogen group and H-estrogen group,systolic blood pressure,diastolic blood pressure,24 h urine protein,Th17 cell ratio,Th17/Treg ratio,IFN-γ,TNF-α,IL-6 and IL-17 levels were significantly decreased,and the proportion of FSH,E2,LH and Treg cells was significantly increased in a dose-dependent manner(P<0.05).Conclusion:Estrogen exposure can reduce the blood pressure of GH rats,improve placental damage and immune function in GH pregnant rats,and the intervention effect of high-dose estrogen is better.

关 键 词：高雌激素 妊娠期高血压 孕鼠 胎盘损伤 免疫功能 

分 类 号：R-33[医药卫生] R714.246