口腔扁平苔藓患者外周血单个核细胞TL1A、sPD-1mRNA表达与Th17/Treg失衡的相关性分析  

Correlation between the Expression of TL1A,sPD-1mRNA in Peripheral Blood Mononuclear Cells and the Imbalance of Th17/Treg in Patients with Oral Lichen Planus

作　　者：杨淇 于洁 曹岩 刘笑萍 郑卫卫 YANG Qi;YU Jie;CAO Yan;LIU Xiao-ping;ZHENG Wei-wei(Department of Ophthalmology and Otorhinolaryngology,The 970 Hospital of Joint Logistics Support Force of Chinese People's Liberation Army,Yantai,Shandong,264001,China)

机构地区：[1]中国人民解放军联勤保障部队第九七〇医院五官科,山东烟台264001

出　　处：《现代生物医学进展》2024年第23期4458-4460,共3页Progress in Modern Biomedicine

基　　金：军队后勤科研基金资助项目(BHJ18J012)。

摘　　要：目的:探讨口腔扁平苔藓(OLP)患者外周血单个核细胞肿瘤坏死因子样配体1A(TL1A)、可溶性程序性死亡受体1(sPD-1)信使核糖核酸(mRNA)表达与辅助性T细胞17(Th17)/调节性T细胞(Treg)失衡的相关性分析。方法:根据133例OLP患者损害类型分为糜烂型组(74例)和非糜烂型组(59例)。检测外周血单个核细胞TL1A mRNA、sPD-1 mRNA表达和外周血维甲酸相关孤核受体γt(RORγt)mRNA、叉头框蛋白P3(FOXP3)mRNA表达以及Th17、Treg细胞比例。Pearson/Spearman相关性分析TL1A mRNA、sPD-1 mRNA表达与Th17/Treg相关因子的关系。结果:与非糜烂型组比较,糜烂型组外周血单个核细胞TL1A mRNA、sPD-1 mRNA、RORγt mRNA表达水平和外周血Th17比例、Th17/Treg比值升高,外周血Treg比例、FOXP3 mRNA表达降低(P<0.05)。相关性分析显示,OLP患者外周血单个核细胞TL1A mRNA、sPD-1 mRNA表达与外周血Th17比例、RORγt mRNA表达、Th17/Treg比值呈正相关,与外周血Treg比例、FOXP3 mRNA表达呈负相关(P<0.05)。结论:OLP患者外周血单个核细胞TL1A、sPD-1异常高表达,与Th17/Treg失衡密切相关,TL1A、sPD-1可能通过引起Th17/Treg失衡参与OLP的发生发展。Objective:To investigate the correlation between the expression of tumor necrosis factor-like ligand 1A(TL1A)and soluble programmed death receptor 1(sPD-1)messenger ribonucleic acid(mRNA)in peripheral blood mononuclear cells and the imbalance of helper T cell 17(Th17)/regulatory T cell(Treg)in patients with oral lichen planus(OLP).Methods:133 patients with OLP(OLP group)were divided into erosive group(74 cases)and non-erosive group(59 cases)according to the type of damage.The expression of TL1A mRNA and sPD-1 mRNA in peripheral blood mononuclear cells,the expression of retinoic acid-related orphan nuclear receptorγt(RORγt)mRNA and forkhead box protein P3(FOXP3)mRNA in peripheral blood,and the proportion of Th17 and Treg cells were detected.The relationship between the expression of TL1A mRNA,sPD-1 mRNA and Th17/Treg related factors were analyzed by Pearson/Spearman correlation analysis.Results:Compared with non-erosive group,the expression levels of TL1A mRNA,sPD-1 mRNA and RORγt mRNA in peripheral blood mononuclear cells,the proportion of Th17 and the ratio of Th17/Treg in peripheral blood were increased,the proportion of Treg and the expression of FOXP3 mRNA in peripheral blood were decreased in erosive group(P<0.05).Correlation analysis showed that,the expression of TL1A mRNA and sPD-1 mRNA in peripheral blood mononuclear cells of OLP patients were positively correlated with the proportion of Th17,the expression of RORγt mRNA and the ratio of Th17/Treg in peripheral blood,and negatively correlated with the proportion of Treg and the expression of FOXP3 mRNA in peripheral blood(P<0.05).Conclusion:The abnormal high expression of TL1A and sPD-1 in peripheral blood mononuclear cells of OLP patients,which are closely related to Th17/Treg imbalance,TL1A and sPD-1 may participate in the occurrence and development of OLP by causing Th17/Treg imbalance.

关 键 词：口腔扁平苔藓 肿瘤坏死因子样配体1A 可溶性程序性死亡受体1 辅助性T细胞17/调节性T细胞失衡 

分 类 号：R758.65[医药卫生—皮肤病学与性病学]