隐丹参酮体外抑制LPS诱导的星形胶质细胞活化  

Study on cryptotanshinone inhibiting LPS-induced astrocytes activation in vitro

作　　者：范祉昀 Fan Zhiyun(Fujian Vocational College of Bioengineering,Fuzhou Fujian 350007)

机构地区：[1]福建生物工程职业技术学院,福建福州350007

出　　处：《山西中医药大学学报》2024年第11期1203-1208,1216,共7页Journal of Shanxi University of Chinese Medicine

基　　金：福建省中青年教师教育科研项目(JAT220634)。

摘　　要：目的:探究隐丹参酮(CTS)在体外对脂多糖(LPS)诱导的星形胶质细胞(AST)活化的影响。方法:体外分离培养小鼠原代AST,先采用CCK-8法检测CTS对AST的细胞毒性,在3~30μmol/L浓度范围内,CTS对小鼠AST没有表现出明显的细胞毒性。后续实验中将AST随机分为对照组、模型组、3个浓度CTS组,CTS给药组分别以3μmol/L、10μmol/L、30μmol/L浓度的CTS对细胞预处理2 h后,再用100 ng/ml LPS刺激24 h,模型组同步用100 ng/ml LPS刺激24 h,对照组常规培养,应用实时荧光定量PCR(real-time PCR)技术检测细胞因子白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)、单核细胞趋化蛋白-1(MCP-1)以及诱导型一氧化氮合酶(iNOS)的mRNA表达水平,以及A1型AST标志性基因H2-T23、H2-D1、Serping1的mRNA表达水平。结果:Real-time PCR检测结果显示,CTS能明显降低IL-6、TNF-α、IL-1β、MCP-1、iNOS的mRNA表达水平以及H2-T23、H2-D1、Serping1的mRNA表达水平,差异有统计学意义(P<0.05)。结论:CTS在体外可有效抑制LPS诱导AST激活,减少细胞因子释放,抑制向A1型AST转化。Objective:To investigate the effect of cryptotanshinone(CTS)on lipopolysaccharide(LPS)-induced activation of astrocytes(AST)in vitro.Methods:Primary mice AST was isolated and cultured in vitro.CCK-8 assay was used to detect the cytotoxicity of CTS to AST.CTS did not show obvious cytotoxicity to mice AST within the concentration range of 3-30μmol/L.In subsequent experiments,the AST were randomly divided into a control group,a model group,and three concentrations CTS groups.The cells in the CTS administration group were pretreated with 3μmol/L,10μmol/L and 30μmol/L CTS for 2 hours,and then stimulated with 100 ng/ml LPS for 24 hours.The cells in the model group were also stimulated with 100 ng/ml LPS for 24 hours,while the cells in the control group were conventionally cultured.Real-time PCR was used to detect the mRNA expression levels of cytokines interleukin-6(IL-6),tumor necrosis factor-alpha(TNF-α),interleukin-1 beta(IL-1β),monocyte chemoattractant protein-1(MCP-1),and inducible nitric oxide synthase(iNOS),as well as the mRNA expression levels of A1 astrocyte marker genes H2-T23,H2-D1,and Serping1.Results:Real-time PCR results showed that CTS significantly reduced the mRNA expression levels of IL-6,TNF-α,IL-1β,MCP-1,iNOS,H2-T23,H2-D1,and the difference was statistically significant(P<0.05).Conclusion:CTS can effectively inhibit LPSinduced AST activation in vitro,reduce cytokine release,and inhibit the conversion to A1 type AST.

关 键 词：星形胶质细胞 隐丹参酮 A1型星形胶质细胞 

分 类 号：R285.5[医药卫生—中药学]