温经散经皮给药的刺激性、过敏性及急性毒性研究  

Study on irritation,anaphylaxis and acute toxicity of percutaneous administration of Wenjing Powder

作　　者：朱丽 罗玲艳 杨康杰 钱海兵 Zhu Li;Luo Lingyan;Yang Kangjie;Qian Haibing(Guizhou University of Traditional Chinese Medicine,Guiyang Guizhou 550025;Key Laboratory of Medicinal Properties and Effects of Traditional Chinese Medicine(Ethnic Medicine),Guizhou University of Higher Learning,Guiyang Guizhou 550025;Key Laboratory of Guizhou Provincial Colleges and Universities,Guiyang Guizhou 550025)

机构地区：[1]贵州中医药大学,贵州贵阳550025 [2]贵州省高等学校中药(民族药)药性与效应研究重点试验室,贵州贵阳550025 [3]贵州省普通高等学校特色重点试验室,贵州贵阳550025

出　　处：《山西中医药大学学报》2024年第11期1217-1223,共7页Journal of Shanxi University of Chinese Medicine

基　　金：贵州省重点科技计划项目(黔科合支撑〔2022〕重点023);贵州省高等学校中药(民族药)药性与效应研究重点试验室项目(黔教技〔2023〕018号)。

摘　　要：目的:探究温经散经皮给药的刺激性、过敏性及急性毒性。方法:(1)刺激性试验:新西兰兔4只,雌雄各半,给药前1 d,用动物剃毛机将新西兰兔脊背两侧对称部位去毛,采用同体左右侧自身对比法,左侧为对照侧,右侧为给药侧,给药剂量为8 g(/kg·d)。给药侧:取备好的温经散均匀涂抹于皮肤,1次/d,连续给药14 d;对照侧:涂抹温水。给药后每天观察新西兰兔的一般生理状况以及皮肤有无红斑和水肿,并进行评分;第14 d给药4 h后空气栓塞法处死新西兰兔进行大体解剖学观察,取给药部位皮肤进行HE染色观察皮肤病理组织学变化。(2)过敏性试验:豚鼠18只,雌雄各半,随机分为对照组、阳性组和温经散组,每组6只。各组在第1天、第7天、第14天致敏,末次致敏后14 d进行1次激发。温经散组豚鼠给药剂量为6 g(/kg·d),阳性组涂抹1.0%2,4-二硝基氯苯(C6H3N2O4Cl),对照组涂抹温水。于激发后1 h,1 d,2 d,3 d观察各组豚鼠的皮肤有无红斑、水肿,并对其进行评分;豚鼠麻醉后颈椎脱臼法处死进行大体解剖学观察。(3)急性毒性试验:大鼠40只,雌雄各半,随机分为对照组和温经散组,每组20只。温经散组给药剂量为6.3 g(/kg·d),对照组大鼠背部两侧涂抹温水,给药后1 d,7 d,14 d记录大鼠体质量变化情况,给药后1 h,1 d,3 d,14 d观察大鼠一般状况及给药部位皮肤状况,第14天给药后麻醉大鼠,腹主动脉取血检测血常规;颈椎脱臼法处死大鼠,进行大体解剖学检查。结果:刺激性试验中新西兰兔给药侧皮肤偶见红斑,无水肿;HE染色显示新西兰兔给药侧和对照侧皮肤结构清晰,无炎性细胞浸润。过敏性试验中阳性组豚鼠过敏现象明显,致敏率为100%,对照组及温经散组豚鼠无过敏现象,致敏率为0。急性毒性试验中,在整个给药期间,两组大鼠的进食量、体质量增长正常,无死亡;大鼠皮肤无红斑、水肿,大体解剖学观察各脏器均未发现�Objective:To investigate the irritation,anaphylaxis and acute toxicity of percutaneous administration of Wenjing Powder.Methods:(1)Irritation test:4 New Zealand rabbits,half male and half female,were selected.One day before administration,the hairs on both sides of the symmetrical parts of the back of New Zealand rabbits were removed.According to the self comparison between the left and right sides of the same body,the left side of the rabbit was the control side,and the right side was the administration side.The dose was 8 g/kg·d.Administration side:the prepared Wenjing Powder was evenly applied to the skin once a day for 14 consecutive days;control side:warm water was applied.The general physiological condition and skin erythema and edema of New Zealand rabbits were observed and scored every day after administration.On the 14th day,4 h after administration,New Zealand rabbits were killed by air embolization for gross anatomical observation,and the skin of the administration site was taken for HE staining to observe the skin histopathological changes.(2)Anaphylaxis test:18 guinea pigs,half male and half female,were randomly divided into control group,positive group and Wenjing Powder group,with 6 in each group.Guinea pigs in each group were sensitized on the 1st,7th and 14th day,and were stimulated once on the 14th day after the last sensitization.The dosage of guinea pigs in Wenjing Powder group was 6 g/kg·d,1.0%C6H3N2O4Cl was applied to guinea pigs in positive group and warm water was applied to guinea pigs in control group.The skin erythema and edema of guinea pigs in each group were observed and scored at 1st h,1st d,2nd d and 3rd d after stimulation.The guinea pigs were killed by cervical dislocation after anesthesia for gross anatomical observation.(3)Acute toxicity test:40 rats,half male and half female,were randomly divided into control group and Wenjing Powder group,with 20 in each group.The administration dose of the rats in Wenjing Powder group was 6.3 g/kg·d,and the rats in control group were coat

关 键 词：温经散 刺激性 过敏性 急性毒性 

分 类 号：R285.5[医药卫生—中药学]