Nanoencapsulation of antioxidant peptides from Lupinus mutabilis in chitosan nanoparticles obtained by ionic gelling and spray freeze drying intended for colonic delivery  

作　　者：Arturo Intiquilla Karim Jiménez-Aliaga Amparo Iris Zavaleta Alexander Gamboa Nelson Caro Mario Diaz Martin Gotteland Lilian Abugoch Cristian Tapia 

机构地区：[1]Laboratorio de Biología Molecular,Facultad de Farmacia y Bioquímica,Universidad Nacional Mayor de San Marcos,Jirón Puno,1002,Lima 1,Peru [2]Departamento de Ciencia de los Alimentos y Tecnología Química,Facultad de Ciencias Químicas y Farmacéuticas,Universidad de Chile,Santos Dumont 964,Santiago,Chile [3]Centro de investigación Austral Biotech,Universidad de Santo Tomas,Santiago de Chile,Chile [4]Facultad de Odontología,Universidad de Chile,Santiago,Chile [5]Departamento de Nutrición,Facultad de Medicina,Universidad de Chile,Chile.y Laboratorio de Microbiología y Probióticos,Instituto de Nutrición y Tecnología de Alimentos (INTA),Universidad de Chile,Chile y Núcleo Milenio en la Biología de la Microbiota Intestinal,Santiago,Chile

出　　处：《Food Bioscience》2022年第6期603-613,共11页食品生物科学（英文）

基　　金：financial support from the“Consejo Nacional de Ciencia,Tecnología e Innovaci´on Tecnol´ogica”(CONCYTEC),Perú(Financial Agreement Number 007-2014-FONDECYT-Perú);financing of research stays from the“Red de Macrouniversidades de Am´erica Latina y el Caribe”Banco Santander and the Doctorate Program in Nutrition and Food at the University of Chile.

摘　　要：Oxidative stress has long been associated with the pathogenesis of inflammatory bowel disease(IBD),and it has been suggested that the combined administration of antioxidants and anti-inflammatory agents may be helpful for its treatment.Biopeptides from plant proteins,such as soybean glycinin tripeptide VPY,effectively inhibit pro-inflammatory mediators in intestinal epithelial and immune cells,which are both involved in the pathogenesis of IBD.This study aimed to produce antioxidant biopeptides from proteins of Lupinus mutabilis seeds and then encapsulate them in chitosan nanoparticles(NPs)for colonic delivery.An antioxidant peptide fraction of less than three kDa(UF3)was obtained and added at different concentrations(0.1–0.4 mg/ml)in chitosan solutions.The NPs were prepared by gelation with tripolyphosphate(CTPP-UF3)or spray freeze-drying of the chitosan solution(SFDC-UF3).Sizes of 332±13 and 465±58 nm and maximum encapsulation efficiencies of 63.80 and 71.75%,respectively,were obtained.UF3 maintained its antioxidant capacity(>80%)and showed different release profiles in 1X PBS buffer at pH 7.4,depending on the encapsulation method.FT-IR showed hydrogen bonding and electrostatic interactions between the peptide and chitosan.Both nanosystems maintained cell viability greater than 70%in colonic cell lines HT-29.These results show that both methods are appropriate for the nanoencapsulation of UF3 and can be used to design nanoparticles for colonic delivery.

关 键 词：ANTIOXIDANT Peptidic fraction Lupinus mutabilis chitosan nanoparticles 

分 类 号：TS255[轻工技术与工程—农产品加工及贮藏工程]