Synergistic effect of oregano essential oil fumigation combined with infrared heating on the inactivation of Aspergillus flavus  

作　　者：Mengmeng Ji Jinwei Li Liuping Fan 

机构地区：[1]State Key laboratory of Food Science&Technology,Jiangnan University,1800 Lihu Avenue,Wuxi,Jiangsu,214122,China [2]School of Food Science and Technology,Jiangnan University,1800 Lihu Avenue,Wuxi,Jiangsu,214122,China [3]Collaborat Innovat Ctr Food Safety&Qual Control,Jiangnan University,1800 Lihu Avenue,Wuxi,Jiangsu,214122,China

出　　处：《Food Bioscience》2022年第6期1735-1745,共11页食品生物科学（英文）

基　　金：financial support of the Key R&D Plan of Xinjiang Uygur Autonomous Region,China(2022B02026-5);National Key Research&Development Plan,China(2018YFC1603705-03);which has enabled us to accomplish this study.

摘　　要：This study was aimed to evaluate the fungicidal effects of oregano essential oil (OEO) fumigation combined with intermediate-wave infrared (IWIR) heating on Aspergillus flavus (A. flavus ) in peanuts, and investigate the synergistic mechanism of the combined treatment (OEO-IWIR). Vitro experiments results indicated that OEO fumigation completely inhibited the A. flavus mycelium growth and aflatoxin production at a dose of 135 mg/L for 6 h. Meanwhile, the sterilization efficacy was assessed by inoculating peanuts with 6 log10  CFU/g A. flavus spores. OEO-IWIR exhibited synergistic effects and reached the 5-log reduction requirement by OEO fumigation at 135 mg/L for 6 h combined with IWIR heating at 65℃ for 1 h. Next, cultivability and spore viability tests of A. flavus were assayed. IWIR heating caused culturable sublethal spores, while the OEO-IWIR exhibited complete inactivation. Finally, the mechanism study confirmed that OEO-IWIR caused a higher degree of cell membrane permeabilization (from 17.0% to 65.6%), more significant alteration of cellular ultrastructure, and higher inhibition of ATPase activity than single treatments.

关 键 词：Aspergillus flavus Essential oil FUMIGATION Infrared heating SYNERGISTIC INACTIVATION 

分 类 号：TQ654.2[化学工程—精细化工]