肝细胞生长因子、成纤维细胞生长因子2和磷脂酰肌醇3-激酶/蛋白激酶B通路在子宫内膜异位症中的表达及其临床意义  

Expression and clinical significance of hepatocyte growth factor,fibroblast growth factor 2 and phosphatidylinositol 3-kinase/protein kinase B pathways in endometriosis

作　　者：王天慧 侯岩岩 叶国柳 Wang Tianhui;Hou Yanyan;Ye Guoliu(Department of Obstetrics and Gynecology,The First Affiliated Hospital of Bengbu Medical University,Bengbu 233000,China)

机构地区：[1]蚌埠医科大学第一附属医院妇产科,蚌埠233000

出　　处：《中国综合临床》2024年第6期437-442,共6页Clinical Medicine of China

摘　　要：目的探讨肝细胞生长因子(hepatocyte growth factor, HGF)、成纤维细胞生长因子2(fibroblast growth factor, FGF-2)和磷脂酰肌醇3-激酶/蛋白激酶B(phosphatidylinositol 3-kinase/protein kinase B, PI3K/AKT)通路在子宫内膜异位症患者表达及相关性。方法收集蚌埠医科大学第一附属医院2023年4月至2024年4月80例经腹腔镜手术获取卵巢巧克力囊肿囊壁组织样本作为异位组, 并选取同期65名正常子宫内膜在位组织样本作为对照组, 通过免疫组化分析HGF、FGF-2和PI3K/AKT在两组中的表达, 分析三者相关性及与EMs患者的临床病理特征的关系。正态分布的计量数据以xˉ±s表示, 两组间均数比较采用独立样本t检验;两变量间的相关性采用Spearman相关分析。采用多因素Logistic回归模型分析子宫内膜异位症患者复发的影响因素。P<0.05为差异有统计学意义。结果 (1)异位组和对照组患者的一般资料(年龄、身高、体质量、体质量指数、囊肿大小)差异均无统计学意义(t值分别为1.81、0.99、0.83、0.95、1.90, P值分别为0.072、0.322、0.409、0.345、0.059);(2)HGF、FGF-2和PI3K主要集中表达于细胞质中, 其中异位组患者的HGF、FGF-2和PI3K阳性率分别为78.75%(63/80)、61.25%(49/80)、71.25%(57/80), 对照组患者的阳性率分别为58.46%(38/65)、38.46%(25/65)、47.69%(31/65), 两组比较差异均有统计学意义(χ^(2)值分别为6.98、7.45、7.44, P值分别为0.008、0.006、0.006);(3)HGF、FGF-2和PI3K表达与EMs患者的临床分期相关(χ^(2)值分别为6.17、4.30、8.65, P值分别为0.013、0.038、0.003), 而与患者年龄、体质量指数、囊肿大小、生育史、月经周期、痛经程度和居住地无关(均P>0.05);(4)异位组患者的HGF与FGF-2表达呈正相关(r=0.590, P<0.05), HGF与PI3K表达呈正相关(r=0.750, P<0.05), FGF-2与PI3K表达呈正相关(r=0.629, P<0.05);(5)EMs患者复发因素的多因素Logistic分析显示, FGF-2是EMs患者复发的保护因素(OR=ObjectiveTo investigate the expression and correlation of hepatocyte growth factor(HGF),fibroblast growth factor 2(FGF-2),and phosphatidylinositol 3-kinase/protein kinase B(PI3K/AKT)pathway in patients with endometriosis.Methods Eighty cases of ovarian chocolate cyst wall tissue samples obtained through laparoscopic surgery at the First Affiliated Hospital of Bengbu Medical University from April 2023 to April 2024 were collected as the ectopic group,and 65 normal endometrial tissue samples from the same period were selected as the control group.The expression of HGF,FGF-2,and PI3K/AKT in both groups was analyzed by immunohistochemistry,and the correlation between the three and the clinical and pathological characteristics of EMs patients was analyzed.The metric data of normal distribution is represented by x¯±s,and the comparison of means between two groups is conducted using independent sample t-test.The correlation between the two variables was analyzed using Spearman correlation analysis.Using a multiple Logistic regression model to analyze the influencing factors of recurrence in patients with endometriosis.P<0.05 indicates a statistically significant difference.Results(1)There was no significant difference in the general data(age,height,body mass,body mass index,cyst size)between the ectopic group and the control group(t values were 1.81,0.99,0.83,0.95,and 1.90,respectively;P values were 0.072,0.322,0.409,0.345,and 0.059,respectively).(2)HGF,FGF-2 and PI3K were mainly expressed in cytoplasm,among which the positive rates of HGF,FGF-2 and PI3K in ectopic group were 78.75%(63/80),61.25%(49/80)and 71.25%(57/80),respectively.The positive rates of the control group were 58.46%(38/65),38.46%(25/65)and 47.69%(31/65),respectively,and there was significant difference between the two groups(χ^(2) values were 6.98,7.45,and 7.44,respectively;P values were 0.008,0.006,0.006,respectively).(3)The expressions of HGF,FGF-2 and PI3K were correlated with the clinical stage of EMs patients(χ^(2) values were 6.17,4.30,and 8.

关 键 词：子宫内膜异位症 磷脂酰肌醇3-激酶/蛋白激酶B 肝细胞生长因子 成纤维细胞生长因子2 

分 类 号：R711.71[医药卫生—妇产科学]