浒苔酶解液用于改善头皮敏感症状的安全性及有效性评估  

Safety and efficacy of enteromorpha prolifera enzymatic hydrolysate in improving scalp sensitive symptoms

作　　者：孙茜 于钰 秦绪艳 冷晓杰 郑玮清[2] Sun Xi;Yu Yu;Qin Xuyan;Leng Xiaojie;Zheng Weiqing(Innovation Laboratory of Qingdao Youdo Bioengineering Co.,Ltd.,Qingdao 266000,China;Department of Dermatology,Qingdao Hiser Hospital Affiliated to Qingdao University,Qingdao 266000,China)

机构地区：[1]青岛优度生物工程有限公司生物研发实验室,青岛266000 [2]青岛大学附属青岛市海慈医院皮肤科,青岛266000

出　　处：《中华医学美学美容杂志》2024年第6期541-546,共6页Chinese Journal of Medical Aesthetics and Cosmetology

基　　金：青岛市2022年度医药卫生科研指导项目(2022-WJZD050)。

摘　　要：目的评估浒苔酶解液在改善头皮敏感症状方面的安全性及有效性。方法通过体外实验观察不同浓度浒苔酶解液对肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1α、IL-6等炎症因子的抑制作用,分析其对[2,2'-联氮-双(3-乙基苯并噻唑啉-6-磺酸)](ABTS)的清除能力,并进行小鼠成纤维细胞促增殖能力测试。前瞻性纳入2023年4至10月青岛大学附属青岛市海慈医院头皮敏感患者20例,其中男2例,女18例,年龄(34.9±10.3)岁。患者在头皮不适处外涂含10%浒苔酶解液的头皮精华液进行治疗,连续使用28 d。分别在使用前及使用后14、28 d测量患者头皮角质层含水量、经皮失水量(TEWL)以及油脂含量,并进行患者满意度及安全性评估。结果体外实验结果显示,不同浓度浒苔酶解液对IL-1α、IL-6、TNF-α表达均有抑制作用,能剂量依赖性清除ABTS自由基,可以增强小鼠成纤维细胞增殖,当浒苔酶解液浓度为0.10%时,小鼠成纤维细胞活力提高43.22%。临床试验结果显示,与使用前相比,在使用含浒苔酶解液的头皮精华液14 d和28 d后,患者头皮角质层含水量提高,TEWL值下降,油脂含量下降,差异均有统计学意义(均P<0.001)。使用含浒苔酶解液的头皮精华液28 d后,治疗头皮泛红、头皮屑、头皮瘙痒、头皮刺痛感、头皮紧绷感的有效率分别为80.0%(16/20)、80.0%(16/20)、85.0%(17/20)、90.0%(18/20)、80.0%(16/20)。20例患者在治疗后均未出现任何不良反应。结论浒苔酶解液在改善头皮敏感症状方面安全有效。ObjectiveTo evaluate the safety and efficacy of enteromorpha prolifera enzymatic hydrolysate in alleviating scalp sensitive symptoms.MethodsThe inhibitory effects of different concentrations of enteromorpha prolifera hydrolysate on tumor necrosis factor(TNF)-α,interleukin(IL)-1α,IL-6,and other inflammatory factors were observed through in vitro experiments.Subsequently,the abilities to scavenge[2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)](ABTS)and to promote proliferation of mouse fibroblasts were evaluated.A prospective study was conducted on 20 patients with scalp sensitivity at Qingdao Hiser Hospital Affiliated to Qingdao University from April 2023 to October 2023.The patients included 2 males and 18 females with an average age of(34.9±10.3)years.They were treated with a scalp essence containing 10%enteromorpha enzymatic hydrolysate for consecutive 28 days.Changes in scalp cuticle water content,transepidermal water loss(TEWL),and oil content were measured before and after 14 and 28 days of treatment.Expert interviewers assessed the skin irritation test to elucidate any undesirable side effects.ResultsThe results of in vitro experiments demonstrated that enteromorpha protophora enzymatic hydrolysate at different concentrations exhibited inhibitory effects on the expression of IL-1α,IL-6,and TNF-α,while it also displayed a dose-dependent ability to scavenge ABTS free radicals.Furthermore,it effectively promoted the proliferation of mouse fibroblasts,resulting in a significant increase of 43.22%in fibroblast viability when the concentration of enteromorpha protophora enzymatic hydrolysate was 0.10%.The outcomes from clinical trials revealed that after using a scalp essence containing enteromorpha protophora enzymatic hydrolysate for 14 and 28 days,there were significant improvements observed in terms of increased water content in the scalp epidermis,along with decreased oil content and TEWL value(all P<0.001).Moreover,remarkable effective rates were achieved for treating various scalp conditions i

关 键 词：头皮 浒苔酶解液 头皮屏障 功效评价 

分 类 号：R62[医药卫生—整形外科]