寻常痤疮患者痤疮丙酸杆菌亚型与表皮屏障功能的相关性研究  

Cutibacterium acnes phylotypes and its association with epidermal barrier function in patients with acne vulagris

作　　者：蒋诗雨 何渊民 黄煜坤 熊霞 邓永琼 Jiang Shiyu;He Yuanmin;Huang Yukun;Xiong Xia;Deng Yongqiong(Department of Dermatology,Chengdu Fifth People's Hospital,Chengdu 611130,China;Department of Dermatology,the Affiliated Hospital of Southwest Medical University,Luzhou 646000,China;Department of Dermatology,Chengdu First People's Hospital,Chengdu 610095,China)

机构地区：[1]成都市第五人民医院皮肤科,成都611130 [2]西南医科大学附属医院皮肤科,泸州646000 [3]成都市第一人民医院皮肤科,成都610095

出　　处：《中华医学美学美容杂志》2024年第6期551-555,共5页Chinese Journal of Medical Aesthetics and Cosmetology

基　　金：四川省科技厅重点研发项目(2019YFS0253);西南医科大学校级基金(2018-ZRQN-002)。

摘　　要：目的分析寻常痤疮患者皮肤表面痤疮丙酸杆菌亚型与表皮屏障功能的相关性。方法前瞻性纳入2018年11月至2019年5月西南医科大学附属医院皮肤科寻常痤疮患者60例,其中男23例,女37例,年龄(21.6±2.6)岁;根据痤疮综合分级系统(GAGS)评分将患者分为3组:轻度28例,中度14例,重度及以上18例。纳入同期面对社会招募的18~30岁健康对照者20名,包括男5名,女15名,年龄(24.2±2.3)岁。采用VISIA皮肤检测仪检测所有受试者面部红色区和紫质百分数。用CK多功能皮肤测试仪检测面部角质层含水量、经皮失水量、pH值、皮肤表面油脂含量。采用16s rDNA测序分析痤疮丙酸杆菌亚型(RT)。各亚型丰度与表皮屏障功能的相关性采用Person相关性分析。结果痤疮患者组与健康对照组相比,紫质百分比[(41.69±23.10)%比(61.33±24.27)%,t=2.98,P<0.01]、红色区百分比[(26.71±17.13)%比(67.97±15.22)%,t=10.78,P<0.01]均下降。与健康对照者比较,不同严重程度痤疮患者角质层含水量均下降,而经皮失水量、皮肤pH值、皮肤表面油脂含量均增加(均P<0.05)。在健康对照组和不同严重程度痤疮患者组中发现了6种不同亚型(RT1、RT2、RT7、RT12、RT13、RT14),上述亚型丰度在健康对照组和轻度痤疮患者组间差异均无统计学意义(均P>0.05);随着痤疮严重程度的增加,以上亚型丰度表现出变化的趋势,RT1丰度逐渐减少,RT2、RT7、RT12、RT13、RT14丰度逐渐增加(均P<0.05)。RT1丰度分别与pH值(r=-0.33,P=0.04)及GAGS评分(r=-0.39,P=0.01)呈负相关。RT2丰度与GAGS评分呈正相关(r=0.39,P=0.02)。RT7丰度与GAGS评分呈正相关(r=0.39,P=0.01)。RT13丰度与角质层含水量呈负相关(r=-0.34,P=0.02),与经皮失水量呈正相关(r=0.30,P=0.05)。结论随着痤疮严重程度的增加,痤疮患者皮肤表面痤疮丙酸杆菌各亚型丰度表现出逐渐升高或降低的趋势。差异痤疮丙酸杆菌亚型丰度与表皮屏障功能密切�ObjectiveTo analyze the correlation between Cutibacterium acnes(C.acne)ribotypes(RTs)on the skin surface and epidermal barrier function in patients with acne vulgaris.MethodsSixty patients with acne vulgaris were enrolled in this prospective study from the Department of Dermatology,the Affiliated Hospital of Southwest Medical University from November 2018 to May 2019,including 23 males and 37 females,aged(21.6±2.6)years.According to the General Acne Grading System(GAGS)score,the patients were divided into three groups:28 mild cases,14 moderate cases,and 18 severe or above cases.Twenty healthy controls aged 18 to 30 years were recruited in the same period from the community,including 5 males and 15 females,aged(24.2±2.3)years.The VISIA skin testing device was used to detect the percentage of red areas and porphyrins on the facial skin of all subjects.The hydration of the stratum corneum(SCH),transepidermal water loss(TEWL),PH value and the skin surface sebum level on the facial skin were detected by CK system.The RTs of C.acne were further analyzed based on the 16s rDNA sequences.The correlation between the abundance of each RT and epidermal barrier function was analyzed using Pearson correlation analysis.ResultsCompared with the healthy controls,the percentage of porphyrins[(41.69±23.10)%vs(61.33±24.27)%,t=2.98,P<0.01]and the percentage of red area[(26.71±17.13)%vs(67.97±15.22)%,t=10.78,P<0.01]decreased in the acne vulgaris patients.Meanwhile,the patients of different severity levels had decreased SCH(P<0.01),but increased TEWL,pH value,and the skin surface sebum level(P<0.05)compared to healthy controls.Six different RTs(RT1,RT2,RT7,RT12,RT13 and RT14)were found in both healthy controls and acne vulgaris patients with varying degrees of severity,while there was no statistically significant difference of C.acne abundance between the mild patients and the controls(P>0.05).As the severity of disease increased,the abundance of the above RTs showed a changing trend,with the gradually decreasing abundance of RT1

关 键 词：寻常痤疮 痤疮丙酸杆菌 菌群亚型 皮肤屏障 16S rRNA 

分 类 号：R758.733[医药卫生—皮肤病学与性病学]