龙血竭提取物调控HIF-1α/VEGF信号通路促进肛周脓肿大鼠创面愈合  

Resina Draconis Extract Regulating HIF-1α/VEGF Signaling Pathway to Promote Wound Healing in Rats with Perianal Abscess

作　　者：王帅 郭金玲 赵雪芳 冯江敏 尤明辉 王立波 王光娜 赵娜 李希 张坤 WANG Shuai;GUO Jinling;ZHAO Xuefang(The Second Affiliated Hospital of Hebei University of Traditional Chinese Medicine,Hebei Dingzhou 073000,China)

机构地区：[1]河北中医药大学第二附属医院,河北定州073000 [2]河北中医药大学第一附属医院内分泌科,河北石家庄050000

出　　处：《河北医学》2024年第12期1958-1965,共8页Hebei Medicine

基　　金：河北省中医药管理局2023年度中医药类科学研究课题,(编号:2023307)。

摘　　要：目的:分析龙血竭提取物调控HIF-1α/VEGF信号通路促进肛周脓肿大鼠创面愈合的影响。方法:选取40只SPF级SD大鼠,10只作为对照组,30只构建肛周脓肿模型,共有24只大鼠建模成功,分为模型组8只、龙血竭提取物组8只、龙血竭提取物+HIF-1α抑制剂组8只,对照组、模型组不进行处理,龙血竭提取物组将龙血竭提取物涂于无菌纱布,覆盖于创面,龙血竭提取物+HIF-1α抑制剂组采用龙血竭提取物敷于创面,并注射10μmoL/L HIF-1α抑制剂。给药14d后,观察各组大鼠病理组织学、创面愈合、新生毛细血管数、成纤维细胞数、血管生成相关因子、炎症因子、HIF-1α/VEGF通路相关mRNA表达量、HIF-1α/VEGF表达量。结果:与模型组相比,龙血竭提取物组、龙血竭提取物+HIF-1α抑制剂组给药4d、9d、14d创面愈合率较高,且龙血竭提取物组创面愈合率较高(P<0.05)。与对照组相比,模型组新生毛细血管数、成纤维细胞数减少,bFGF、EGF水平、HIF-1α、VEGF相关mRNA表达量、HIF-1α、VEGF表达量降低,TNF-α、IL-6、IL-β水平升高,与模型组相比,龙血竭提取物组、龙血竭提取物+HIF-1α抑制剂组新生毛细血管数、成纤维细胞数增加,bFGF、EGF水平、HIF-1α、VEGF相关mRNA表达量、HIF-1α、VEGF表达量升高,TNF-α、IL-6、IL-β水平降低,且龙血竭提取物组新生毛细血管数、成纤维细胞数多于龙血竭提取物+HIF-1α抑制剂组,bFGF、EGF水平、HIF-1α、VEGF相关mRNA表达量、HIF-1α、VEGF表达量高于龙血竭提取物+HIF-1α抑制剂组,TNF-α、IL-6、IL-β水平低于龙血竭提取物+HIF-1α抑制剂组(P<0.05)。结论:采用龙血竭提取物对肛周脓肿大鼠干预,可通过调控HIF-1α/VEGF信号通路,促使大鼠创面愈合,具有较好的使用效果。Objective:To analyze the effect of resina draconis extract on promoting wound healing in rats with perianal abscess by regulating HIF-1α/VEGF signaling pathway.Methods:Forty SPF grade SD rats were selected,with 10 as the control group and 30 used to construct the perianal abscess model.A total of 24 rats were successfully modeled and divided into a model group(8),a resina draconis extract group(8),and a resina draconis extract+HIF-1αinhibitor group(8).The control group and the model group were not treated.The resina draconis extract group was treated with resina draconis extract coated on sterile gauze and covered on the wound surface,while the resina draconis extract+HIF-1αinhibitor group was treated with resina draconis extract applied on the wound surface and injected with 10μmoL/L HIF-1αinhibitor.After 14 days of administration,the pathological histology,wound healing,number of newly formed capillaries,fibroblast count,angiogenesis related factors,inflammatory factors,HIF-1α/VEGF pathway related mRNA expression levels,and HIF-1α/VEGF expression levels were observed in each group of rats.Results:Compared with the model group,the resina draconis extract group and the dragon's blood extract+HIF-1αinhibitor group had higher wound healing rates after 4 days,9 days,and 14 days of treatment,and the resina draconis extract group had a higher wound healing rate(P<0.05).Compared with the control group,the number of newly formed capillaries and fibroblasts in the model group decreased,while the levels of bFGF,EGF,HIF-1α,VEGF related mRNA expression,HIF-1α,and VEGF expression decreased.The levels of TNF-α,IL-6,and IL-βincreased.Compared with the model group,the resina draconis extract group and the resina draconis extract+HIF-1αinhibitor group increased the number of newly formed capillaries and fibroblasts,while the levels of bFGF,EGF,HIF-1α,VEGF related mRNA expression,HIF-1α,and VEGF increased.The levels of TNF-α,IL-6,and IL-βdecreased,and the number of newly formed capillaries and fibroblasts in the

关 键 词：肛周脓肿 龙血竭提取物 缺氧诱导因子-1α/血管内皮生长因子 创面愈合 

分 类 号：R285.5[医药卫生—中药学]