microRNA-138通过调控FBLN5/IL-1β途径缓解大鼠盆腔器官脱垂  

microRNA-138 Alleviates Pelvic Organ Prolapse in Rats by Regulating FBLN5/IL-1βPathway

作　　者：袁梦玮 晏梓宴 毛怡 赵新[1] YUAN Mengwei;YAN Ziyan;MAO Yi(Chengdu Third People's Hospital,Sichuan Chengdu 610031,China)

机构地区：[1]四川省成都市第三人民医院康复医学科,四川成都610031

出　　处：《河北医学》2024年第12期1971-1978,共8页Hebei Medicine

基　　金：2021年四川省医学(青年创新)科研课题,(编号:S21931)。

摘　　要：目的:分析microRNA-138通过调控FBLN5/IL-1β途径缓解大鼠盆腔器官脱垂的机制研究。方法:选取40只自然分娩过3次的SPF级SD雌性大鼠,其中10只作为对照组,另外30只构建盆腔器官脱垂模型,麻醉后撑开大鼠阴道将导尿管缝合于阴道口,使其下垂,2周进行双侧卵巢切除术,共有27只大鼠建模成功,分为模型组9只、miRNA-138 inhibitor组9只、miRNA-138 inhibitor+FBLN5抑制剂组9只,对照组、模型组注射生理盐水,miRNA-138 inhibitor组注射10μL miR-138inhibitor慢病毒悬液,miRNA-138 inhibitor+FBLN5抑制剂组注射10μL miR-138inhibitor+10μL FBLN5抑制剂。观察各组大鼠病理组织学、成纤维细胞数量、尿动力学、Ⅰ型胶原、Ⅲ型胶原表达量、FBLN5/IL-1β相关mRNA表达量、FBLN5/IL-1β表达量。结果:与对照组相比,模型组成纤维细胞数量减少,膀胱基础压、膀胱排尿量、膀胱排尿压、膀胱峰值压、Ⅰ型胶原、Ⅲ型胶原表达量、FBLN5相关mRNA表达量、FBLN5表达量降低,IL-1β相关mRNA表达量、IL-1β表达量升高,与模型组相比,miRNA-138 inhibitor组、miRNA-138 inhibitor+FBLN5抑制剂组成纤维细胞数量增加,膀胱基础压、膀胱排尿量、膀胱排尿压、膀胱峰值压、Ⅰ型胶原、Ⅲ型胶原表达量、FBLN5相关mRNA表达量、FBLN5表达量升高,IL-1β相关mRNA表达量、IL-1β表达量降低,且miRNA-138 inhibitor组成纤维细胞数量较多,膀胱基础压、膀胱排尿量、膀胱排尿压、膀胱峰值压、Ⅰ型胶原、Ⅲ型胶原表达量、FBLN5相关mRNA表达量、FBLN5表达量较高,IL-1β相关mRNA表达量、IL-1β表达量较低(P<0.05)。结论:抑制microRNA-138表达,可调控FBLN5/IL-1β途径,促使成纤维细胞、胶原蛋白的表达,并改善大鼠尿动力学,缓解盆腔器官脱垂。Objective:To analyze the mechanism of microRNA-138 in alleviating pelvic organ prolapse in rats by regulating the FBLN5/IL-1βpathway.Methods:Forty SPF grade SD female rats with three natural childbirths were selected,of which 10 were used as the control group,and another 30 were used to construct a pelvic organ prolapse model.After anesthesia,the vagina of the rats was opened and the urinary catheter was sutured to the vaginal opening to make it droop.Two weeks later,bilateral oophorectomy was performed.A total of 27 rats were successfully modeled and divided into model group(n=9),miRNA-138 inhibitor group(n=9),and miRNA-138 inhibitor+FBLN5 inhibitor group(n=9).The control group and model group were injected physiological saline,the miRNA-138 inhibitor group was injected 10μL of miR-138 inhibitor lentivirus suspension,and the miRNA-138 inhibitor+FBLN5 inhibitor group was injected 10μl of miR-138inhibitor lentivirus suspension.inhibitor+10μL FBLN5 inhibitor.The histopathological changes were observed,as well as fibroblast count,urodynamics,expression levels of type I and typeⅢcollagen,FBLN5/IL-1β-related mRNA expression levels,and FBLN5/IL-1βexpression levels in each group of rats.Results:Compared with the control group,the number of fibroblasts in the model decreased,and the basal bladder pressure,bladder voiding volume,bladder voiding pressure,bladder peak pressure,expression levels of type I collagen,typeⅢcollagen,FBLN5 related mRNA expression,and FBLN5 expression decreased.The expression levels of IL-1β-related mRNA and IL-1βincreased.Compared with the model group,the miRNA-138 inhibitor group and miRNA-138 inhibitor+FBLN5 inhibitor group had an increase in the number of fibroblasts and an increase in bladder basal pressure,bladder voiding volume,bladder voiding pressure,bladder peak pressure,type I collagen,typeⅢcollagen expression,FBLN5 related mRNA expression,and FBLN5 expression.However,the expression of IL-1β-related mRNA and IL-1β-related mRNA decreased,and miRNA-138 inhibitor group had a

关 键 词：盆腔器官脱垂 微小RNA-138 衰老关键蛋白5/白介素-1β 胶原蛋白 

分 类 号：R711.2[医药卫生—妇产科学]