优质小麦品种温麦10号籽粒蛋白质组学分析  

Proteomic analysis of grain of high-quality wheat variety Wenmai 10

作　　者：刘永安 黄业昌 岳高红 高锡腾 邓立章 潘彬荣 LIU Yongan;HUANG Yechang;YUE Gaohong;GAO Xiteng;DENG Lizhang;PAN Binrong(South Zhejiang Key Laboratory of Crop Breeding,Wenzhou 325006,Zhejiang,China;Wenzhou Academy of Agricultural Sciences,Wenzhou 325006,Zhejiang,China)

机构地区：[1]浙南作物育种重点实验室,浙江温州325006 [2]温州市农业科学研究院,浙江温州325006

出　　处：《浙江农业学报》2024年第11期2437-2446,共10页Acta Agriculturae Zhejiangensis

基　　金：“十四五”浙江省农业(旱粮新品种选育)新品种选育重大科技专项(2021C02064-3);温州市农业新品种选育协作组项目(2019ZX006-5)。

摘　　要：为探究温麦10号成为优质素面小麦品种的原因,以温麦10号和扬麦20(对照)为材料,采用非标记定量蛋白质组学(Label-free)技术结合生物信息学分析法,对差异表达蛋白质进行鉴定并对其进行功能分析。结果表明,两个小麦品种中共鉴定出78个显著性差异表达蛋白质,其中温麦10号有40个蛋白质上调,38个蛋白质下调。同时,还鉴定出207个有无差异表达蛋白质,其中107个蛋白质只在温麦10号籽粒中表达,100个蛋白质只在扬麦20籽粒中表达。对差异表达蛋白质进行功能分析,表明高(低)分子量谷蛋白亚基、醇溶蛋白及类燕麦贮藏蛋白可能是影响温麦10号和扬麦20品质的重要因素。In order to explore the reasons why Wenmai 10 became a high-quality wheat variety of hand-made dry noddles,Wenmai 10 and Yangmai 20(control)were used as materials and Label-free quantitative proteomics(label-free)technology combined with bioinformatics analysis was used to identify and perform functional analysis of different-expressed proteins.The results showed that a total of 78 differentially expressed proteins were identified between the two wheat varieties,of which 40 proteins were up-regulated and 38 proteins were down-regulated in Wenmai 10.At the same time,207 proteins presence/absence differential expression were also identified,of which 107 proteins were only expressed in Wenmai 10 and 100 proteins were only expressed in Yangmai 20.The functional analysis of different-expressed proteins showed that high(low)molecular weight glutenin subunits,gliadin and avenin-like proteins might be important factors affecting the quality of Wenmai 10 and Yangmai 20.

关 键 词：温麦10号 小麦 蛋白质组 素面 

分 类 号：S512.1[农业科学—作物学]