番鸭A-FABP基因外显子2多态性及其与生长性能和肉品质的关联分析  

Study on correlation of polymorphism of A-FABP gene exon 2 with growth performance and meat quality in Muscovy duck

作　　者：董飚[1] 纪荣超 张干生 王健[1,2] DONG Biao;JI Rongchao;ZHANG Gansheng;WANG Jian(Jiangsu Agri-Animal Husbandry Vocational College,Taizhou 225300,Jiangsu,China;National Gene Bank of Waterfowl Resources,Taizhou 225300,Jiangsu,China)

机构地区：[1]江苏农牧科技职业学院,江苏泰州225300 [2]国家级水禽基因库,江苏泰州225300

出　　处：《浙江农业学报》2024年第11期2456-2464,共9页Acta Agriculturae Zhejiangensis

基　　金：江苏高校“青蓝工程”(苏教师函[2021]11号)。

摘　　要：为了了解A-FABP基因第2外显子单核苷酸多态在黑羽番鸭群体中的分布情况,以及这些单核苷酸多态与性能的关联性,文章采用PCR-SSCP技术检测了黑羽番鸭A-FABP基因第2外显子多态性,分析了该群体遗传参数,以及多态性与生长性能、肉品质的关联性,以期为分子标记辅助选择提供参考。结果表明,黑羽番鸭A-FABP基因第2外显子上存在A-C突变,为同义突变。形成了AA型、AB型和BB型3种基因型,基因型频率分别为0.098 8、0.401 2和0.500 0,A等位基因频率为0.299 4,B等位基因频率为0.700 6。群体纯合度为0.580 5,杂合度为0.419 5,多态信息含量为0.331 5。AA型公鸭初生体重显著高于AB型和BB型。公鸭AA型腹脂重显著高于AB型和BB型,AA型腺肌胃重显著高于BB型。母鸭AB型、BB型的半净膛率显著性高于AA型,AA型腹脂重显著高于BB型,AA型腹脂率显著高于AB型和BB型。公鸭胸肌AA型pH值显著高于AB型和BB型。AA型和AB型公鸭胸肌中肌苷酸含量显著高于BB型,AB型天冬氨酸含量显著高于AA型。BB型母鸭胸肌中谷氨酸含量显著高于AA型。AA型公鸭腿肌中谷氨酸、甘氨酸含量显著高于AB型和BB型。AA型母鸭腿肌中甘氨酸含量显著高于AB型和BB型,AA型母鸭腿肌中苏氨酸、缬氨酸含量显著高于BB型。因此,A-FABP基因外显子2中的A-C可用于黑羽番鸭腹脂重和肌肉部分氨基酸含量的辅助选择。The study was to investigate the distribution of single nucleotide in the A-FABP gene exon 2 in black Muscovy duck,and the correlation between these single nucleotide and performance.The polymorphism of exon 2 of A-FABP gene was detected by PCR-SSCP technique in black Muscovy duck.The genetic parameters of this population and the polymorphism with growth performance and meat quality were analyzed,so as to provide reference for molecular marker-assisted selection.The result indicated that there was A-C mutation in exon 2 of A-FABP gene about black Muscovy duck,which was synonymous mutation.Three genotypes,AA,AB and BB,were formed with genotype frequencies of 0.0988,0.4012 and 0.5000,A and B allele frequencies of 0.2994 and 0.7006,respectively.The homozygosity,heterozygosity and polymorphic information content(PIC)was 0.5805,0.4195 and 0.3315,respectively.The birth weight of AA male duck was significantly higher than that of AB and BB male duck.The abdominal fat weight of AA male duck was significantly higher than that of AB and BB,and the stomachus muscularis weight of AA male duck was significantly higher than that of BB.The percentage of half-eviscerated yield of AB and BB was significantly higher than that of AA,the abdominal fat weight of AA was significantly higher than that of BB,and the percentage of abdominal fat of AA was significantly higher than that of AB and BB in female duck.The pH value in breast muscle of AA male duck was significantly higher than that of AB and BB.The content of inosinic acid in breast muscle of AA and AB males was significantly higher than that of BB males,and the content of Asp in AB males was significantly higher than that of AA males.The content of Glu in breast muscle of BB female duck was significantly higher than that of AA type.The content of Glu and Gly in leg muscle of AA male duck was significantly higher than that of AB and BB.The content of Gly in leg muscle of AA female ducks was significantly higher than that of AB and BB,and the content of Thr and Val in leg muscle

关 键 词：黑羽番鸭 A-FABP基因 多态性 生长性能 肉品质 

分 类 号：S834.89[农业科学—畜牧学]