腹泻仔猪肠道菌群结构特征及肠道炎症因子、腺苷酸环化酶和鸟苷酸环化酶mRNA表达变化研究  

Study on structural characteristics of intestinal microflora and expression changes of intestinal inflammatory factors, adenylate and guanylate cyclase mRNA in diarrheal piglets

作　　者：吴兴凤 章啸君 朱江 余敏洁 徐娥 马灵燕 肖英平[2] WU Xingfeng;ZHANG Xiaojun;ZHU Jiang;YU Minjie;XU E;MA Lingyan;XIAO Yingping(Key Laboratory of Genetics,Breeding and Reproduction of Plateau Mountain Animals,Ministry of Education,School of Animal Science,Guizhou University,Guiyang 550025,China;State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products,Institute of Agro-Product Safety and Nutrition,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;Institute of Animal Husbandry,Veterinary Medicine and Fisheries,Jinhua Academy of Agricultural Sciences,Jinhua 321017,Zhejiang,China)

机构地区：[1]贵州大学动物科学学院,高原山地动物遗传育种与繁殖教育部重点实验室,贵州贵阳550025 [2]浙江省农业科学院农产品质量安全与营养研究所,省部共建农产品质量安全与危害因子防控国家重点实验室,浙江杭州310021 [3]金华市农业科学研究院畜牧兽医与水产研究所,浙江金华321017

出　　处：《浙江农业学报》2024年第11期2465-2475,共11页Acta Agriculturae Zhejiangensis

基　　金：金华市农业科技重点项目(2021-2-010);国家自然科学基金(31402083,32372907)。

摘　　要：选取同批次的21日龄仔猪,在断奶3 d后采集55份健康仔猪和37份腹泻仔猪的粪便样本,测定短链脂肪酸含量,采用高通量测序技术基于16S rRNA基因分析菌群结构,并分别选择6头腹泻和健康仔猪屠宰采集结肠黏膜样品对炎症因子、腺苷酸环化酶和鸟苷酸环化酶的mRNA表达水平进行检测。结果显示,与健康仔猪相比,腹泻仔猪粪便菌群的香农指数、Chao1指数显著(P<0.05)降低;在门水平上,变形菌门(Proteobacteria)和厚壁菌门(Firmicutes)的相对丰度显著提高,拟杆菌门(Bacteroidota)的相对丰度显著降低;在属水平上,大肠埃希菌-志贺菌属(Escherichia-Shigella)、乳酸杆菌属(Lactobacillus)和拟普雷沃氏菌属(Alloprevotella)的相对丰度显著升高,瘤胃球菌属(Ruminococcus)、瘤胃球菌科_uncultured(Ruminococcaceae_uncultured)、普雷沃氏菌科_NK3B31(Prevotellaceae_NK3B31_group)、理研菌科RC9属(Rikenellaceae_RC9_gut_group)、UCG-010_norank和狭义梭菌属_1(Clostridium_sensu_stricto_1)的相对丰度显著降低。菌群功能预测结果显示,与健康仔猪相比,腹泻仔猪中粪便菌群的糖酵解/糖异生、D-谷氨酰胺和D-谷氨酸代谢、D-精氨酸代谢通路的相对丰度显著升高,细菌趋化性,以及缬氨酸、亮氨酸和异亮氨酸的生物合成(valine, leucine and isoleucine biosynthesis)通路的相对丰度显著降低。腹泻仔猪粪便中的乙酸、丙酸和丁酸含量较健康仔猪显著降低,且乙酸含量与理研菌科RC9属、UCG-010_norank和瘤胃球菌科_uncultured的相对丰度呈显著正相关,丁酸含量与罗氏菌属(Roseburia)、不动杆菌属(Agathobacter)和狭义梭菌属_1的相对丰度呈显著正相关。与健康仔猪相比,腹泻仔猪肠道中促炎因子——肿瘤坏死因子-α(TNF-α)和白细胞介素-8(IL-8)的mRNA表达水平显著升高,抗炎因子——白细胞介素-10(IL-10)的mRNA表达水平显著降低(P<0.05),腺苷酸环化酶mRNA的表达水平显著升高。由此可见,�In the present study,the same batch of 21-day-old piglets were selected.Fecal samples of 55 healthy piglets and 37 diarrhea piglets were collected after 3 days of weaning.The content of short-chain fatty acids was determined,and the microbial community structure was analyzed by high-throughput sequencing of 16S rRNA gene.Six diarrheal and healthy piglets were selected and slaughtered to collect colonic mucosa samples to detect the mRNA expression levels of inflammatory factors,adenylate cyclase and guanylate cyclase.It was shown that the Shannon index and Chao1 index of microbial community in diarrheal weaned piglets were significantly(P<0.05)decreased compared with those in the healthy group.At the phylum level,the relative abundance of Proteobacteria and Firmicutes in the microbial community of diarrhea weaned piglets was significantly increased,yet the relative abundance of Bacteroidota was significantly decreased compared with those of the healthy pigles.At the genus level,the relative abundance of Escherichia-Shigella,Lactobacillus and Alloprevotella was significantly increased,yet the relative abundance of Ruminococcus,Ruminococcaceae_uncultured,Prevotellaceae_NK3B31_group,Rikenellaceae_RC9_gut_group,UCG-010_norank and Clostridium_sensu_stricto_1 was significantly decreased than those of the healthy piglets.The prediction of microbial function showed that the relative abundance of glycolysis/gluconeogenesis,D-glutamine and D-glutamate metabolism,and D-alanine metabolism pathways in the microbial community of diarrheal piglets was significantly increased compared with the healthy group,while the relative abundance of bacterial chemotaxis and valine,leucine and isoleucine biosynthesis pathways was significantly reduced.The contents of acetic acid,propionic acid and butyric acid in the intestine of diarrhea piglets were significantly lowered than those in the healthy piglets.The relative abundance of Rikenellaceae_RC9_gut_group,Ruminococcaceae_uncultured and UCG-010_norank flora was significantly positively co

关 键 词：断奶仔猪 腹泻 肠道菌群 腺苷酸环化酶 鸟苷酸环化酶 

分 类 号：S858.28[农业科学—临床兽医学]