Metabolomics combined with network pharmacology reveals anti-asthmatic effects of Nepeta bracteata on allergic asthma rats  

作　　者：Kailibinuer Abulaiti Miheleayi Aikepa Mireguli Ainaidu Jiaxin Wang Maiwulanijiang Yizibula Maihesumu Aikemu 

机构地区：[1]Institute of Traditional Uyghur Medicine,Xinjiang Medical University,Urumqi 830017,China [2]Central Laboratory Institute,Xinjiang Medical University,Urumqi 830011,China

出　　处：《Chinese Herbal Medicines》2024年第4期599-611,共13页中草药（英文版）

基　　金：financially supported by Xinjiang Uygur Autonomous Region Department of Science and Technology (No. 2016A03005-1)。

摘　　要：Objective: To investigate the mechanisms that underlie the anti-asthmatic effects of Nepeta bracteata(DBJJ, Dabao Jingjie in Chinese) in rats by integrating metabolomics and network pharmacology.Methods: In this study, the rat model of asthma was induced by ovalbumin(OVA), and the rats were treated with a decoction of N. bracteata. Pathological changes in lung tissue were observed, and the quantification of eosinophils(EOS) and white blood cells(WBC) in bronchoalveolar lavage fluid was performed.Furthermore, the serum levels of asthma-related factors induced by OVA were assessed.^(1)H NMR spectroscopy serum metabolomics method was utilized to identify differential metabolites and their associated metabolic pathways. UPLC-QE-MS/MS combined with network pharmacology was employed to predict the core targets and pathways of DBJJ in its action against asthma. The anti-asthmatic properties of DBJJ were investigated using an integrated approach of metabolomics and network pharmacology. The findings were validated through molecular docking and Western blotting analysis of the key targets.Results: The administration of DBJJ effectively alleviated OVA-induced lung histopathological changes and decreased the number of EOS and WBC in BALF. Additionally, DBJJ inhibited the OVA-induced elevation of TNF-a, IL-18, Ig-E, EOS, IL-1β, MDA, VEGF-A, and TGF-β1. A total of 21 biomarkers and 10 pathways were found by metabolomics analysis. A total of 29 compounds were identified by UPLC-QE-MS/MS, in which 13 active components were screened by oral availability and Caco-2 cell permeability, the 120 targets and 173 KEGG pathways were predicted. The integration of metabolomics and network pharmacological analysis revealed that DBJJ's main constituents, including ferulic acid and ursolic acid, exerted their effects on four targets, namely DAO and NOS2, as well as their associated metabolites and pathways.The active constituents of DBJJ demonstrated a high binding affinity towards DAO and NOS2.Furthermore, DBJJ was observed to decrease th

关 键 词：allergic asthma MAPK metabolomics network pharmacology Nepeta bracteata Benth. NOS2 STAT3 

分 类 号：R285.5[医药卫生—中药学]