Inula britannica ameliorates alcohol-induced liver injury by modulating SIRT1-AMPK/Nrf2/NF-κB signaling pathway  

作　　者：Zhennan Meng Mengyuan Li Xiaoli Wang Kuo Zhang Chunfu Wu Xiaoshu Zhang 

机构地区：[1]Faculity of Functional Food and Wine,Shenyang Pharmaceutical University,Shenyang 110016,China [2]School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China

出　　处：《Chinese Herbal Medicines》2024年第4期667-678,共12页中草药（英文版）

基　　金：supported by the National Natural Science Foundation of China (grant No. 81703389);Natural Science Foundation of Science and Technology Department of Liaoning Province (No. 2021-MS-215);Youth Development Support Plan of Shenyang Pharmaceutical University (No. ZQN2021010)。

摘　　要：Objective: Inula britannica is a traditional Chinese medicinal and functional food with various effects such as anti-liver injury, hypoglycemia, antioxidants, and anti-tumor. The aim of this study was to investigate the protective effects and mechanisms of the ethanolic extract of I. britannica(EEIB) on alcohol-induced liver injury in mice.Methods: Fifty-six female C57BL/6 mice were randomly divided into seven groups: control group(Con),ethanol feeding model group(EtOH), Silibinin positive treatment group(EtOH + Silibinin 100 mg/kg),EEIB treatment group(EtOH + EEIB 100, 200, and 400 mg/kg), and EEIB control group(EEIB 400 mg/kg). The National Institute on Alcohol Abuse and Alcoholism(NIAAA) ethanol-feeding model was used to study the effects of EEIB on alcohol-induced lipid metabolism, inflammation, oxidative stress, and fibril formation in mice by histopathological evaluation, immunofluorescence staining, Western blotting analysis and molecular docking.Results: EEIB reduced liver indices to different degrees to normal levels and improved liver morphology in mice. EEIB inhibited alcohol-induced liver injury by activating the sirtuin 1(SIRT1)-adenosine monophosphate-activated protein kinase(AMPK) signaling pathway in the liver of alcohol-fed mice, in which sesquiterpenes may be the potential active ingredients, and also down-regulated the expression of alpha-smooth muscle actin(a-SMA), collagen alpha(Collagen I), tumor necrosis factor-alpha(TNFa) and attenuated alcohol-induced liver injury. In addition, EEIB also activated the nuclear factor erythroid 2-related factor 2(Nrf2) signaling pathway, which alleviated alcohol-induced liver injury at the level of oxidative stress. Notably, the EEIB control group demonstrated that EEIB had no toxic effects in mice. EEIB reduced alcoholic liver injury in a dose-dependent manner. Its therapeutic efficacy was comparable to, if not better than, that of Silibinin when administered at a dose of 400 mg/kg.Conclusion: EEIB showed significant therapeutic effects on alcohol-induced

关 键 词：alcoholic liver injury inflammation Inula britannica L SESQUITERPENES SIRT1-AMPK/Nrf2 

分 类 号：R285[医药卫生—中药学]