Multi-omics analysis reveals the neuroprotective effect of Atractylodis Rhizoma Alba extract against Parkinson's disease in mouse  

作　　者：KANG Sohi LEE Soong-In MOON Byeong Cheol SONG Jun Ho KIM Sung-Ho MOON Changjong LEE Sueun KIM Chul KIM Joong Sun 

机构地区：[1]Department of Anatomy and Convergence Medical Science,College of Medicine,Institute of Health Sciences,Gyeongsang National University,Jinju 52727,Republic of Korea [2]College of Veterinary Medicine and BK21 FOUR Program,Chonnam National University,Gwangju 61186,Republic of Kore [3]Department of Oriental Medicine Prescription,College of Oriental Medicine,Dong-Shin University,Gwangju 61186,Republic of Kore [4]Herbal Medicine Resources Research Center,Korea Institute of Oriental Medicine,111,Geonjaero,Naju-si,Jeollanam-do 58245,Republic of Korea [5]Department of Biology,Chungbuk National University,Cheongju 28644,Republic of Korea [6]KM Data Division,Korea Institute of Oriental Medicine,1672 Yuseong-daero,Yuseong-gu,Daejeon 34054,Republic of Korea

出　　处：《Journal of Traditional Chinese Medicine》2024年第6期1111-1117,共7页中医杂志（英文版）

基　　金：the Development of Sustainable Application for Standard Herbal Resource by the Korea Institute of Oriental Medicine (No. KSN2012320)。

摘　　要：OBJECTIVE: To assess Atractylodis Rhizoma Alba extract(ARE) neuroprotective function in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-treated mice and related genes. METHODS: Examined m RNA-DNA methylation changes induced by ARE in MPTP-induced Parkinson's disease(PD) model's substantia nigra. RESULTS: ARE mitigated MPTP-induced motor impairment in rotarod and open field tests and preserved tyrosine hydroxylase-positive neuronal cells in substantia nigra and striatum. Genome RNA-Sequencing and Methyl-Sequencing in substantia nigra of vehicle/ARE-treated MPTP-induced PD mice showed 84 differentially expressed genes(DEGs) and 1804 differentially methylated regions(DMRs). Upregulated genes involved zinc ion homeostasis, cilium protein localization, and transcription;downregulated genes linked to ephrin receptor signaling, somitogenesis, and gene expression regulation. Hyper/hypomethylated DMRs post-ARE treatment associated with Wnt signaling, mitochondrial organization, dopamine biosynthesis, and hindbrain development. No significant correlation between DEGs and methylated genes related to PD pathogenesis. CONCLUSION: This research has identified the epigenetic targets of ARE's therapeutic action and gives insight on how ARE protects neurons in Parkinson's disease.

关 键 词：Parkinson disease nerve degeneration 1-methyl-4-phenyl-1 2 3 6-TETRAHYDROPYRIDINE Atractylodis Rhizoma Alba Methyl-seq 

分 类 号：R285.5[医药卫生—中药学]