Bushen Huoxue decoction(补肾活血汤)improves the reproduction of endometriosis-associated infertility by regulating Homeobox A10 and αlpha(v)beta(3)integrin expression  

作　　者：TANG Weiwei LIU Kaili FAN Xumei ZHU Li ZENG Zheng SUN Jiali SHI Jie ZHANG Zhenzhen GUI Tao WAN Guiping 

机构地区：[1]Department of Obstetrics and Gynecology,Affiliated Hospital of Integrated Traditional Chinese and Western Medicine,Nanjing University of Chinese Medicine,Nanjing 210028,China [2]Department of Pathology,Affiliated Hospital of Integrated Traditional Chinese and Western Medicine,Nanjing University of Chinese Medicine,Nanjing 210028,China

出　　处：《Journal of Traditional Chinese Medicine》2024年第6期1137-1145,共9页中医杂志（英文版）

基　　金：National Natural Science Foundation-funded Project:the Mechanism of Effect of Bushen Huoxue Decotion on Endometrial Receptivity in Endometriosis (No. 81603372);National Natural Science Foundation-funded Project:to Investigate the Mechanism of Bushen Huoxue Decoction in Improving Endometrial Receptivity Based on SUMOylation of Homeobox A10 (No. 82274190);Jiangsu Commission of Health Project:to Investigate the Mechanism of Bushen Huoxue Decoction in Improving Endometrial Receptivity Based on Leukemia Inhibitory Factor Signaling (No. M2022079)。

摘　　要：OBJECTIVE: To investigate the mechanism of Bushen Huoxue decoction( 补肾活血汤, BSHXD) to treat endometriosis-induced infertility. MEDHODS: The main compounds of BSHXD were determined by high performance liquid chromatographymass spectrometry(HPLC-MS/MS). The effect of BSHXD on Homeobox A10(HOXA10) and alpha(v)beta(3)(αvβ3) integrin expression of Ishikawa cells, mouse model, and endometriosis-associated infertility women was evaluated by using Western blot analysis, immunohistochemistry and Real-Time quantitative polymerase chain reaction(RTq PCR). The efficacy of BSHXD on embryo attachment were examined by using the Be Wo spheroid and mouse embryo attachment assay. HOXA10 concentration in uterine flushing fluid of endometriosis-associated infertility women treated with BSHXD was measured by EnzymeLinked immunosorbent assay(ELISA).RESULTS: BSHXD improved Be Wo spheroid and mice blastocysts attachment to Ishikawa cells and increased embryo implantation rates in mice and pregnancy rates in women with endometriosis-associated infertility. BSHXD enhanced HOXA10 and αvβ3 integrin expression in Ishikawa cell, endometriosis mouse model, and endometriosis-associated infertility women, which potentially improved endometrial receptivity. CONCLUSIONS: BSHXD could improve endometrial receptivity of endometriosis-associated infertility in a dosedependent manner by regulating HOXA10 and αvβ3 integrin expression.

关 键 词：INFERTILITY ENDOMETRIOSIS homeobox A10 proteins integrin alpha Vbeta3 Bushen Huoxue decoction 

分 类 号：R285.5[医药卫生—中药学]