Huaiyu pill(槐榆片)alleviates inflammatory bowel disease in mice via blocking toll like receptor 4/myeloid differentiation primary response gene 88/nuclear factor kappa B subunit 1 pathway  

作　　者：YANG Chunyan LUO Jia PENG Weijie DAI Weibo 

机构地区：[1]Department of Pharmacy,Zhongshan Hospital of Traditional Chinese Medicine,Guangzhou University of Traditional Chinese Medicine,Zhongshan 528400,China [2]Department of Pharmacy,Shenshan Medical Center Memorial Hospital of Sun Yat-Sen University Sun Yat-Sen University,Shanwei 516600,China

出　　处：《Journal of Traditional Chinese Medicine》2024年第5期916-925,共10页中医杂志（英文版）

基　　金：the Medical Scientific Research Foundation of Guangdong Province of China:Mechanistic Study on the Regulation of Mucin 2/interleukin 6-signal Transducer and Activator of Transcription 3 Signaling Pathway by Active Saponin Ardisiacrispin B in Improving Ulcerative Colitis Intestinal Mucosal Barrier Dysfunction(A2022479);the Guangdong Provincial Basic and Applied Basic Research Project:Mechanistic Study on the Regulation of Inflammatory Microenvironment and Improvement of Ulcerative Colitis by Lingnan Traditional Medicine Ficus Quercifolia through Wilms'tumor 1-associating Protein-Mediated RNA Methyltransferase Promoting Toll Like Receptor 4 m6A Modification(2023A1515011699)。

摘　　要：OBJECTIVE:To investigate the therapeutic effects of Huaiyu pill(槐榆片,HYP)on inflammatory bowel disease(IBD)and the underlying mechanisms have not been elucidated.METHODS:To establish the IBD model,mice were administered with dextran sulfate sodium(DSS).Mice were intragastrically pre-treated with sulfasalazine(SASP)and HYP.Disease activity index(DAI)and colon length were monitored,and the colonic tissues were subjected to hematoxylin-eosin staining.Pro-inflammatory factors and vascular inflammation-related proteins were determined using enzyme-linked immunosorbent assay(ELISA).The potential mechanisms of HYP were examined using network pharmacology analysis.The expressions of zona occludens 1(ZO-1),occludin,toll like receptor 4(TLR4),myeloid differentiation primary response gene 88(MYD88),and nuclear factor kappa B p65 subunit(NF-κB p65)in colon tissues were examined using Western blotting or immunohistochemical analyses.RESULTS:Pre-treatment with HYP enhanced the colon length,decreased DAI scores,and mitigated histopathological alterations in DSS-treated mice.HYP alleviated intestinal inflammation by downregulating the levels of interleukin 1 beta(IL-1β),interleukin 6(IL-6),tumor necrosis factor alpha(TNF-α)and interleukin 17(IL-17).Additionally,HYP suppressed the disruption of the gut barrier by upregulating the ZO-1,occludin,and mucin 2(MUC2)levels and downregulating the endothelin 1(ET-1)and erythropoietin(EPO)levels.Network pharmacological analysis and experimental results revealed that HYP downregulated the colonic tissue levels of TLR4,MYD88,and NF-κB p65 in DSStreated mice.CONCLUSION:This study investigated the in vivo therapeutic effects of HYP on IBD and the underlying molecular mechanisms.These findings provide an experimental foundation for the clinical application of HYP.

关 键 词：inflammatory bowel diseases network pharmacology intestinal inflammation intestinal damage Huaiyu pill 

分 类 号：R285.5[医药卫生—中药学]