Association of miR-499 rs3746444,miR-149 rs2292832 polymorphisms and their expression levels with helicobacter pylori-related gastric diseases and Traditional Chinese Medicine syndromes  

作　　者：LIU Qi YU Chang YE Jintong ZHANG Ling LI Danyan DAI Yunkai ZHANG Yunzhan LUO Qi CHEN Weijing PAN Huaigeng LI Ruliu HU Ling 

机构地区：[1]Institute of Gastroenterology,Science and Technology Innovation Center,Guangzhou University of Chinese Medicine,Guangzhou 510000,China [2]First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510000,China

出　　处：《Journal of Traditional Chinese Medicine》2024年第5期1024-1034,共11页中医杂志（英文版）

基　　金：the National Natural Science Foundation of China:the Exploration of the Traditional Chinese Medicine Syndrome Characteristics During Benign and Malignant Pathological Evolution in Hp-Related Gastric Diseases from Wnt/β-catenin Signaling Pathway based on the Theory of"Evil Poison Lead to Change"(No.81774238);the Exploration of the Traditional Chinese Medicine Syndrome Characteristics During Benign and Malignant Pathological Evolution in Hp-Related Gastric Diseases based on the Theory of"Latent Toxin"and the Polymorphism and Expression of Mucosal Barrier Related Genes(No.82174298)。

摘　　要：OBJECTIVE:To provide an objective experimental basis for the gastric mucosa pathological evolution and the transformation of different Traditional Chinese Medicine(TCM)syndromes in helicobacter pylori(H.pylori)-related gastric diseases(HPGD)patients,based on the combination of TCM syndrome differentiation,molecular biology and histopathology.METHODS:A total of 203 participants were enrolled in this study.The expressions of miR-499/miR-149 and H.pylori infection in the gastric tissues from all participants were detected.The genotyping for miR-499 rs3746444 and miR-149 rs2292832 was performed.RESULTS:In H.pylori positive subjects,the proportion of precancerous gastric lesions(PGL)in liver-stomach disharmony syndrome(LSDS)group was higher than in spleen Qi deficiency syndrome(SQDS)group(P<0.001);The proportion of gastric cancer(GC)in SQDS group was higher than in spleen-stomach damp-heat syndrome(SSDHS)group and LSDS group(all P<0.001).We also found C allele of miR-149 rs2292832 was linked to lower risk of gastric atrophy[miR-149 rs2292832 C vs T:adjusted odds ratio=0.207;95%confidence interval(0.043-0.989);P=0.048].Compared with healthy control(HC)group,the expression of miR-499 was significantly increased in GC group,while the expression of miR-149was significantly decreased in chronic inflammation group,PGL group and GC group(all P<0.05).Test for trend showed that GC risk was on a rising trend with the increasing expression of miR-499 and decreasing expression of miR-149(both P for trend<0.05).CONCLUSION:The C allele of miR-149 rs2292832 may be a protective factor for gastric mucosal atrophy.H.pylori may participate in the evolution of benign to malignant gastric mucosa lesions by inducing the overexpression of miR-499 and down regulation of miR-149.In addition,patients with H.pylori infection combined SQDS or LSDS may have higher risk of gastric mucosal malignant lesions.

关 键 词：MICRORNAS helicobacter pylori stomach diseases POLYMORPHISM genetic syndrome complex 

分 类 号：R259[医药卫生—中西医结合]