基于ITS2基因对海南岛热带环境白纹伊蚊的分子鉴定  

Molecular ITS2 gene identification of Aedes albopictus in the tropical environment of Hainan Island

作　　者：徐艺嘉 冯行之 王玉铌 李明玉 夏乾峰 芦亚君 XU Yi-jia;FENG Xing-zhi;WANG Yu-ni;LI Ming-yu;XIA Qian-feng;LU Ya-jun(NHC Key Laboratoryof Tropical Disease Control,School of Tropical Medicine,Hainan Medical University,Haikou 571199,China;School of Basic Medical Sciences,Xi’an Jiaotong University,Xi’an 710049,China)

机构地区：[1]国家卫生健康委员会热带病防治重点实验室,海南医科大学热带医学院,海口571199 [2]西安交通大学基础医学院,西安710049

出　　处：《中国人兽共患病学报》2024年第11期1003-1008,共6页Chinese Journal of Zoonoses

基　　金：国家自然科学基金(No.82360028)。

摘　　要：目的通过对海南岛白纹伊蚊ITS2基因的分子鉴定,分析岛内白纹伊蚊的遗传多样性、单倍型特征及系统发育关系,以揭示海南岛白纹伊蚊种群的遗传结构。方法采集海南岛18个行政市(县)白纹伊蚊,提取基因组DNA并扩增ITS2基因。使用MEGA 11.0软件分析碱基组成,计算遗传距离,并构建系统发育关系。使用DnaSP 6软件和PopArt 1.7软件计算单倍型数量,绘制单倍型拓扑网络图,并分析单倍型多样性。结果获得海南岛18个市(县)白纹伊蚊共计360条ITS2基因,序列长度为536~586 bp,GC含量为51.29%~55.31%,平均种内遗传距离为0.0154,共存在251个单倍型,单倍型多样性指标为0.9870,具有丰富的遗传多样性和错综复杂的亲缘关系。海南岛白蚊伊蚊与洪都拉斯白纹伊蚊的系统发育关系最近。结论海南岛白纹伊蚊种群内部存在广泛的基因交流和丰富的遗传多样性,为种群的进化和适应提供了充足的基因资源。Through molecular identification of the ITS2 gene of Aedes albopictus in Hainan Island,we analyzed the genetic diversity,haplotype characteristics,and phylogenetic relationships,to characterize the genetic structure of the local Aedes albopictus population.Aedes albopictus samples were collected from 18 administrative cities and counties in Hainan Island.Genomic DNA was extracted,and the ITS2 gene was amplified.The base composition was analyzed in MEGA 11 software.The genetic distances were calculated,and the phylogenetic relationships were constructed.DnaSP6 and PopArt 1.7 software were used to calculate the number of haplotypes,draw a haplotype topology network diagram,and analyze haplotype diversity.A total of 360 ITS2 genes were obtained from Aedes albopictus in 18 cities and counties in Hainan Island,with sequence lengths ranging from 536 to 586 bp;GC content ranging from 51.29%to 55.31%;average intraspecies genetic distances ranging from 0.0154;a total of 251 haplotypes and a haplotype diversity index of 0.9870,indicating rich genetic diversity and intricate kinship relationships,the phylogenetic relationship between Aedes albopictus in Hainan Island and in Honduras was the closest.The existence of extensive gene exchange and rich genetic diversity within the Aedes albopictus population in Hainan Island provided sufficient genetic resources for the evolution and adaptation of the population.

关 键 词：海南岛 白纹伊蚊 ITS2基因 遗传多样性 系统发育关系 分子鉴定 

分 类 号：R384.1[医药卫生—医学寄生虫学]