2020-2023年山西省布鲁氏菌分子流行病学特征分析  

Molecular epidemiological characteristics of Brucella in Shanxi Province from 2020 to 2023

作　　者：王洋[1] 姚素霞[1] 郝瑞娥[1] 韩吉婷 张秋香[1] 杨红霞[1] WANG Yang;YAO Su-xia;HAO Rui-e;HAN Ji-ting;ZHANG Qiu-xiang;YANG Hong-xia(Shanxi Provincial Key laboratory for Major Infectious Disease Response,Shanxi Provincial Center for Disease Control and Prevention,Taiyuan 030012,China)

机构地区：[1]山西省疾病预防控制中心,重点传染性疾病预防与诊治山西省重点实验室,太原030012

出　　处：《中国人兽共患病学报》2024年第11期1031-1035,共5页Chinese Journal of Zoonoses

基　　金：山西省卫生健康委科研课题(No.2022156)。

摘　　要：目的分析2020-2023年山西省布鲁氏菌分子流行病学特征。方法对2020-2023年山西省临床分离的479株布鲁氏菌进行常规生物学鉴定,提取基因组进行MLVA-16分型和全基因组测序。结果多位点可变数串联重复序列分析(Multipk-Locus Variable-number-Analysis,MLVA)聚类显示总体相似度在50.5%~100%,共204种VNTR型,聚类结果5株以上的成簇VNTR型有11种,其中有1种成簇数量在25株的VNTR型和1种成簇数量在17株的VNTR型。对这2种VNTR型的全部菌株进行全基因组测序,测序结果分别进行核心基因组多位点序列分型(Core Genenome Multilocus Sequence Typing,cgMLST)聚类,存在成簇的菌株,且差异位点较少。结论MLVA分型方法可将布鲁氏菌进行有效分辨,cgMLST能进一步阐述菌株亲缘关系和分子流行病学特征。山西省内存在着特定基因型的布鲁氏菌的长期传播。This study was aimed at analyzing the molecular epidemiological characteristics of Brucella in Shanxi Province from 2020 to 2023.A total of 479 clinical Brucella strains were identified in Shanxi Province from 2020 to 2023.The strains were preliminarily identified through biochemical methods.The genome was extracted for MLVA-16 typing and whole genome sequencing.MLVA-16 clustering results indicated 50.5%to 100%strain similarity.The strains were typed into 204 VNTR types by MLVA-16,whereas the panel 2B locus showed polymorphism.The clustering results indicated 11 VNTR types with more than five strains.Whole genome sequencing was performed for VNTRS types in large clusters.cgMLST clustering indicated similar clustering of strains.Brucella was effectively distinguished through MLVA typing.The phylogenetic relationship and epidemiological characteristics of strains can be distinguished more effectively by cgMLST.Specific Brucella genotypes were transmitted in Shanxi Province from 2020 to 2023.

关 键 词：布鲁氏菌 分子分型 多位点可变数串联重复序列分析 核心基因组多位点序列分型 

分 类 号：R378.5[医药卫生—病原生物学]