2012-2023年湖北省人间布鲁氏菌株的药敏谱和病原学特征分析  

Drug sensitivity spectrum and pathogenic characteristics of human Brucella strains in Hubei Province from 2012 to 2023

作　　者：专行 陈平[2] 张雅婷 李国明[1] 江无悔 戴莹[1] ZHUAN Hang;CHEN Ping;ZHANG Ya-ting;LI Guo-ming;JIANG Wu-hui;DAI Ying(Hubei Provincial Center for Disease Control and Prevention,Wuhan 430079,China;Suizhou Center for Disease Control and Prevention,Suizhou 441300,China)

机构地区：[1]湖北省疾病预防控制中心,武汉430079 [2]随州市疾病预防控制中心,随州441300

出　　处：《中国人兽共患病学报》2024年第11期1036-1041,共6页Chinese Journal of Zoonoses

基　　金：国家科技重大专项(No.2018ZX10201-002)基金资助。

摘　　要：目的了解湖北省人间布鲁氏菌的药敏谱和病原学特征。方法对2012-2023年湖北省所分离的122株人间布鲁氏菌株进行回顾性分析。采用分离培养、生化鉴定、分子生物学方法、血清学鉴定等方法进行病原学鉴定,采用E-test方法进行体外药敏试验,采用全基因组测序方法对选取25株布鲁氏菌进行MLST分型和系统进化发育树分析。结果本研究中的布鲁氏菌均为羊种3型。122株布鲁氏分离株药敏结果显示109株布鲁氏菌分离株对12种药物普遍敏感,13株布鲁氏菌对11种药物敏感,对复方新诺明中介。MLST 9位点序列分型显示25株布鲁氏分离菌均为ST8型,毒力基因特征相似,但不同菌株所携带的毒力基因略有差别。系统进化发育树分析结果显示25株布鲁氏分离菌可分成2个大进化分支和多个亚分支,呈现多态性。结论湖北省流行的人间布鲁氏菌以ST8型为优势克隆群,并且携带多种免疫逃逸相关毒力基因,复方新诺明的MIC值升高,需加强监测。This study was aimed at investigating the antimicrobial susceptibility spectrum and pathogenic characteristics of human brucellosis in Hubei Province.A retrospective analysis was performed on 122 brucellosis cases reported in Hubei Province from 2012 to 2023.Isolation and culture,biochemical identification,molecular biology methods,and serological identification were used for pathogen identification;the E-test method was used for in vitro drug susceptibility testing;and whole genome sequencing was used to perform MLST typing and phylogenetic developmental tree analysis of 25 strains of Brucella.All Brucella isolates in Hubei Province were Brucella melitensis bv.3 str.The drug susceptibility results for 122 Brucella isolates indicated that 109 Brucella isolates were generally sensitive to 12 drugs,among which 13 Brucella isolates were sensitive to 11 drugs,and were intermediate to sulfamethoxazole.MLST 9-site sequence typing indicated that all 25 strains of Brucella isolates were ST8 type,with similar virulence gene characteristics,but different strains carried slightly different virulence genes.Phylogenetic tree analysis demonstrated that the 25 strains of Brucella isolates could be divided into two major evolutionary branches and multiple subbranches,and exhibited polymorphism.The dominant clone group of human Brucella in Hubei Province was ST8 type,which carried multiple virulence genes associated with immune escape.The MIC for sulfamethoxazole has increased,and surveillance should be strengthened.

关 键 词：布鲁氏菌 种型鉴定 体外药敏试验 MLST分型 遗传进化分析 

分 类 号：R378.5[医药卫生—病原生物学]