Sanhuang Xiexin Decoction Ameliorates TNBC by Modulating JAK2-STAT3 and Lipid Metabolism  

作　　者：QI Ying WU Xin-jie SHI Jing-bin SHI Xiao-wei ZHAO Na XIONG Yang WANG Li-pei 

机构地区：[1]School of Basic Medical Sciences,Hangzhou Normal University,Hangzhou 311121,China [2]Key Laboratory of Aging and Cancer Biology of Zhejiang Province,Institute of Aging Research,Hangzhou Normal University School of Medicine,Hangzhou 311121,China [3]Zhejiang Chinese Medical University,Hangzhou 310053,China

出　　处：《Chinese Journal of Integrative Medicine》2024年第12期1080-1089,共10页中国结合医学杂志（英文版）

基　　金：Supported by Natural Science Foundation of Zhejiang Province(No.LY20C060002)。

摘　　要：Objective:To investigate the therapeutic effect of Sanhuang Xiexin Decoction(SXD)on triplenegative breast cancer(TNBC)in mice and its underlying mechanism.Methods:The high-performance liquid chromatography(HHLC)was used to quantitate and qualify SXD.A total of 15 female BALB/c mice were inoculated subcutaneously on the right hypogastrium with 3×10^(5) of 4T1-Luc cells to establish TNBC mouse model.All mice were divided randomly into 3 groups,including phosphate buffered solution(PBS),SXD and doxorubicin(DOX)groups(positive drug).Additionally,tumor growth,pathological changes,serum lipid profiles,expression of Janus kinase 2(JAK2)-signal transducer and activator of transcription 3(STAT3)signaling pathway and its key targets including inflammatory factors,cell cycle and epithelial-mesenchymal transition(EMT)markers were investigated.Besides,the biosafety of SXD was also evaluated in mice.Results:Rhein,coptisine,berberine hydrochloride and baicalin were all found in SXD,and the concentrations of these 4 components were 0.57,2.61,2.93,and 46.04 mg/g3respectively.The mouse experiment showed that SXD could notably suppress the development of tumors and reduce the density of tumor cells(P<0.01).The serum lipid analysis and Oil-Red-O staining both showed the differences,SXD group exhibited higher serum adiponectin and HDL-C levels with lower TC and LDL-C levels compared to the PBS and DOX groups(P<0.05 or P<0.01),respectively.SXD also decreased the levels of phospho-JAK2(p-JAK2),phospho-STAT3(p-STAT3)expressions and its downstream factors,including mostly inflammatory cytokine,EMT markers,S phase of tumor cells and vascular endothelial growth factor(VEGF)expression(P<.05 or P<0.01),respectively.The biosafety assessment of SXD revealed low levels of toxicity in mice.Conclusion:SXD could inhibit TNBC by suppressing JAK2-STAT3 phosphorylation which may be associated with modulation of lipid metabolism.

关 键 词：Sanhuang Xiexin Decoction Janus kinase 2-signal transducer and activator of transcription 3 lipid metabolism triple-negativebreast cancer epithelial-mesenchymal transition 

分 类 号：R285[医药卫生—中药学]