HPLC测定兽用穿琥宁注射液中的有关物质  

Determination of the Related Substances of Potassinm Veterinary Dehydro and Rographolide Succinate Injection by HPLC

作　　者：章文丽 李鑫 马春花 余佳欣 杨涵月 李诗 董可 ZHANG Wen-li;LI XIN;MA Chun-hua;YU Jia-xin;YANG Han-yue;LI Shi;DONG Ke(Sichuan Yuqiang Herbal Biotechnology Co.,Ltd.,Chengdu 61100,China)

机构地区：[1]四川育强本草生物技术有限公司,成都611100

出　　处：《中国兽药杂志》2024年第12期9-16,共8页Chinese Journal of Veterinary Drug

摘　　要：建立一种HPLC法测定兽用穿琥宁注射液中的有关物质。采用Inertsil ODS-35μm(4.6 mm×250 mm)色谱柱;以0.1%磷酸二氢钾溶液(用磷酸调节pH值至2.5±0.1)为流动相A,以乙腈为流动相B,梯度洗脱;检测波长为251 nm。该方法专属性、稳定性、耐用性、重现性等良好,灵敏度高。该方法适用于兽用穿琥宁注射液中有关物质的测定。To establish an HPLC method to determine the relevant substances in veterinary water injection.Use Inertsil ODS-35μm(4.6 mm×250 mm)column;use 0.1%potassium dihydrogen phosphate solution(adjust pH with phospic acid to 2.5±0.1)as mobile phase A,acetonitrile as mobile phase B;gradient elution;detection wavelength is 251 nm.Results:The method has good specificity,stability,durability,reproducibility and high sensitivity.This method is suitable for the determination of related substances in animal water injection.

关 键 词：兽用穿琥宁注射液 有关物质 高效液相色谱法 

分 类 号：S859.83[农业科学—临床兽医学]