UPLC-PDA联合UPLC-MS/MS法确证一批标称白头翁口服液中非法添加物恩诺沙星  

UPLC-PDA Combined UPLC-MS/MS Method to Confirm the Illegal Addition of Enrofloxacin in One Batch of Nominal Baitouweng Koufuye

作　　者：魏秀丽 张琦 邵兵 刘少宁 刘华阳 张传津 杨志昆 李有志 WEI Xiu-li;ZHANG Qi;SHAO Bing;LIU Shao-ning;LIU Hua-yong;ZHANG Chan-jin;YANG Zhi-kun;LI You-zhi(Shandong Center for Quality Control of Feed and Veterinary Drug,Shandong Provincial Key Laboratory of Quality Safety Monitoring for Animal Products and Veterinary Drug Innovation,Jinan 250100,China;Shandong Livestock Product Quality and Safety Center,Jinan 250100,China)

机构地区：[1]山东省饲料兽药质量检验中心,山东省畜产品质量安全监测与新兽药创制重点实验室,济南250100 [2]山东省畜产品质量安全中心,济南250100

出　　处：《中国兽药杂志》2024年第12期17-26,共10页Chinese Journal of Veterinary Drug

基　　金：泰山产业创新领军人才专项资助(TSCX202211023);山东省重点研发计划“蛋鸡福利化健康养殖与用药安全关键技术研究”(2022CXPT022)。

摘　　要：采用UPLC-PDA联合UPLC-MS/MS方法确证了一批标称白头翁口服液中非法添加物恩诺沙星;样品经提取稀释后,采用Waters BEH C 18色谱柱为分离柱,UPLC-PDA初步筛查,再用ACQUITY UPLC T 3色谱柱分离,液相色谱串联质谱仪采用正离子扫描方式,上机测定。通过对比色谱图保留时间和光谱图的峰形以及质谱图,结果表明最终确证该批样品中非法添加物为恩诺沙星。本方法快速、灵敏、重现性好,适用于本批标称白头翁口服液中恩诺沙星的检测。A mothod was established for confirmation of enrofloxacin in a batch of Baitouweng koufuye by UPLC-PDA joint UPLC-MS/MS..After extraction and dilution,waters BEH C18 column as separation column,UPLC-PDA preliminary screening,and then separated by ACQUITY UPLC T3 chromatography column,liquid chromatography tandem mass spectrometer by positive ion scanning and measurement.By comparing the retention time of the chromatogram and the peak shape of the spectrogram and the mass spectrometer,the results showed that the illegal addition was enrofloxacin.This method is fast,sensitive and reproducible,and is suitable for the detection of enrofloxacin in this batch of nominal Baitouweng koufuye.

关 键 词：白头翁口服液 恩诺沙星 非法添加 

分 类 号：S859.83[农业科学—临床兽医学]