姜黄提取物对加州鲈生长、抗氧化及免疫功能的影响  

Effect of Curcuma longa Extracts on Growth,Antioxidant and Immune Functions of Micropterus salmoides

作　　者：余少梅 姚秀荣[1,2] 罗杨志 刘欣苑 胡少迪 邓平 YU Shao-mei;Yao Xiu-rong;LUO Yang-zhi;LIU Xin-yuan;HU Shao-di;DENG Ping(Wuhan Academy of Agriculture Science&Technology,Wuhan 430207,China;Wuhan Chopper Fishery Bio-Tech Co.,Ltd.,Wuhan 430207,China;Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]武汉市农业科学院,武汉430207 [2]武汉中博水产生物技术有限公司,武汉430207 [3]华中农业大学,武汉430070

出　　处：《中国兽药杂志》2024年第12期48-57,共10页Chinese Journal of Veterinary Drug

基　　金：武汉市农业科学院学科创新专项(XKCX202303-2、XKCX202403-2);国家特色淡水鱼产业技术体系(CARS-46);武汉市科技特派员产学研专项(2023110201030682);湖北省农业科技创新中心创新团队项目(2024-620-000-001-033)。

摘　　要：为探究姜黄提取物对加州鲈生长、抗氧化及免疫功能的影响,在饲料中分别添加1%的姜黄醇提物和姜黄水提物,进行4周的投喂试验,每周测定加州鲈体质量及肝脏抗氧化指标,试验结束时测定脾脏炎性因子及抗病毒因子的基因表达量。结果显示,投喂姜黄醇提取物和水提物对加州鲈幼鱼体质量增长无显著影响(P>0.05)。醇提物组SOD、AKP在第3周开始显著高于对照组(P<0.05),第4周时显著高于水提物组(P<0.05),第4周时CAT、T-AOC、ACP显著高于对照组及水提物组(P<0.05),MDA显著低于对照组及水提物组(P<0.05)。姜水提物组SOD在第3周开始显著高于对照组(P<0.05),第4周时T-AOC、ACP显著高于对照组(P<0.05),MDA及CAT显著小于对照组(P<0.05)。用药组加州鲈脾脏中IL-8、IL-10、TNF-α、IFN-γ、Mx-1和PKR基因表达量均显著高于对照组(P<0.05),其中醇提物组IL-10、Mx-1和PKR基因表达量显著高于水提物组(P<0.05),水提物组IFN-γ基因表达量较醇提物组高(P<0.05)。研究表明,饲料中添加适量的姜黄醇提物和水提物,投喂4周能显著提高加州鲈抗氧化能力及免疫力,以姜黄醇提物效果更优。In order to study the effects of Curcuma longa extracts on the growth,antioxidant capacity and immunity of Micropterus salmoides,the experimental groups were fed with diets containing ethanol or water extracts of Curcuma longa at the level of 1%for 4 weeks.Body weight and antioxidant indexes in the liver were measured weekly,and the gene expression of inflammatory factors and antiviral proteins in the spleen were detected at the end of the experiment.The results showed that dietary supplementation of Curcuma longa extracts had no significant influence on body weight(P>0.05).In the group fed with ethanol extract,the SOD and AKP activities were significantly higher than those in the control group from the third week(P<0.05),and were significantly higher than those in the group fed with water extract at the fourth week(P<0.05).The CAT,T-AOC and ACP activities in the group fed with ethanol extract were significantly higher than those in other groups(P<0.05),while MDA content was significantly lower than that in other groups at the fourth week(P<0.05).In the group fed with water extract,the SOD activity was significantly higher than that in the control group from the third week(P<0.05).The T-AOC and ACP activities in the group fed with water extract were significantly higher than those in the control group(P<0.05),while the MDA content and CAT activity were significantly lower than those in the control group at the fourth week(P<0.05).The gene expression levels of IL-8,IL-10,TNF-α,IFN-γ,Mx-1 and PKR in the two groups fed with Curcuma longa extracts were dramatically higher than those in the control group(P<0.05).The gene expression levels of IL-10,Mx-1,and PKR in the ethanol extract group were significantly higher than those in the water extract group(P<0.05),while the gene expression level of IFN-γin the water extract group was higher than that in the ethanol extract group(P<0.05).These findings indicated that a diet supplemented with Curcuma longa extracts for 4 weeks can markedly enhance the antioxidant capacity

关 键 词：姜黄醇提物 姜黄水提物 生长 抗氧化 免疫 

分 类 号：S859.79[农业科学—临床兽医学]