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作 者:Zihang Shi Xiankang Fan Maolin Tu Zhen Wu Daodong Pan
机构地区:[1]State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products,Ningbo University,Ningbo,Zhejiang,315211,China [2]Key Laboratory of Animal Protein Food Processing Technology of Zhejiang Province,College of Food and Pharmaceutical Sciences,Ningbo University,Ningbo,315832,China
出 处:《Food Bioscience》2023年第1期498-507,共10页食品生物科学(英文)
基 金:supported by the National Natural Science Foundation of China(NSFC31972048,NSFC32272339).
摘 要:As the healthy diet concept has gained broad acceptance,more and more consumers nowadays prefer to drink fermented milk.Indeed,the quality of fermented milk is influenced by the protein hydrolysis capacity of the bacterial strains used.In this study,we conducted an in silico analysis of the composition of the proteolytic system in the genomes of Lactobacillus helveticus R0052 and Lactococcus lactis subsp.lactis JCM5805,and assessed the impact of these two strains on the quality of fermented milk from several perspectives.The results showed that L.helveticus R0052 and L.lactis JCM5805 had different cell envelope proteases,oligopeptides ABC transport system,and peptidases in their protein hydrolysis systems,which resulted in significant differences in protein hydrolysis,pH,acidity,viable bacteria count,water holding capacity and texture of the produced fermented milk.Differences in the metabolic profiles and pathways of volatile and non-volatile flavor substances between L.helveticus R0052 and L.lactis JCM5805 were identified by metabolomics.L.helveticus R0052 improves the sensory quality of fermented milk compared to L.lactis JCM5805.Our study findings provide novel insights into selecting suitable strains to produce fermented milk from a genetic and metabolic perspective.
关 键 词:Lactic acid bacteria Fermented milk Proteolytic system Metabolomics
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