‘红太阳’墨兰不同叶色时期的转录组和代谢组联合分析  

Joint Analysis of Transcriptome and Metabolome at Different Leaf Color Stages of Cymbidium sinense‘Red Sun’

作　　者：高洁 相楚嫣 赵俊宏 潘启明 杨凤玺 GAO Jie;XIANG Chuyan;ZHAO Junhong;PAN Qiming;YANG Fengxi(Environmental Horticulture Research Institute,Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization,Guangzhou 510640,China;School of Pharmaceutical Sciences,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Institute of Facility Agriculture,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;Zhaoqing Yalanfang Agricultural Technology Co.,Ltd.,Zhaoqing 526200,China)

机构地区：[1]广东省农业科学院环境园艺研究所/广东省园林花卉种质创新与利用重点实验室,广东广州510640 [2]广州中医药大学中药学院,广东广州510006 [3]广东省农业科学院设施农业研究所,广东广州510640 [4]肇庆雅兰芳农业科技有限公司,广东肇庆526200

出　　处：《广东农业科学》2024年第10期17-30,共14页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(31902065);国家重点研发计划项目(2023YFD2300904);广东省农业科学院科技创新战略专项(高水平农科院建设)(R2021PY-QF003,ZX202402);广东省乡村振兴战略专项种业振兴行动项目(2023-NBH-00-009);广东省科技计划项目(2023B0202010030)。

摘　　要：【目的】探明‘红太阳’墨兰不同叶色变化过程中相关基因的变化模式,为墨兰叶色改良提供指导。【方法】利用Illumina HiSeq高通量测序平台对‘红太阳’墨兰红色、黄色以及绿色叶片进行测序,分析差异表达基因的富集通路,同时对转录组和代谢组数据进行联合分析,并对筛选出的关键差异基因进行荧光定量PCR的验证。【结果】从红叶向黄叶转变过程中一共筛选到861个差异基因(DEGs),其中上调基因数为204个、下调基因数为657个;从黄叶向绿叶转变过程中一共筛选到815个差异基因,其中上调基因有492个、下调基因有323个。KEGG通路富集分析结果显示,与色素合成和代谢相关通路上的基因在‘红太阳’墨兰叶色变化过程中均出现显著富集。在叶色转变过程中,MYB、NAC、WRKY以及bHLH转录因子家族的表达量出现显著性差异。与代谢组数据联合分析发现,黄酮类、异黄酮类化合物、黄酮和黄酮醇以及代谢物合成途径中的差异基因和差异代谢物在‘红太阳’墨兰叶色变化过程中显著富集。类黄酮代谢途径中共鉴定出26个差异表达基因,这些基因在红叶向黄叶转变过程中均表现出显著下调,5种差异代谢物的含量在这一转变过程中显著下降。在黄叶向绿叶的转变中,17个基因和2种代谢物(新橙皮苷和柚皮苷查尔酮)表现出显著差异。【结论】花青苷合成前体物质含量以及相关合成酶基因表达量的降低导致‘红太阳’墨兰叶片红色逐渐消失,光合色素合成相关基因的高表达是导致‘红太阳’墨兰叶色由黄最后变绿的主要原因。【Objective】The study aims to elucidate the change patterns of related genes during the color transition of Cymbidium sinense‘Red Sun’,in order to provide guidance for the improvement of C.sinense leaf color.【Method】The Illumina HiSeq high-throughput sequencing platform was used to sequence the red,yellow and green leaves of C.sinense‘Red Sun’,analyze the enrichment pathway of differentially expressed genes,and conduct a joint analysis of transcriptome and metabolome data.Key differential genes were also verified by fluorescent quantitative PCR.【Result】The results showed that a total of 861 differentially expressed genes(DEGs)were screened during the transition from red to yellow leaves,with 204 up-regulated genes and 657 down-regulated genes;during the transition from yellow to green leaves,a total of 815 DGEs were screened,with 492 up-regulated genes and 323 down-regulated genes.KEGG pathway enrichment analysis revealed that genes related to pigment synthesis and metabolism were significantly enriched during the color transition process of C.sinense‘Red Sun’.During the leaf color transition,the expression levels of transcription factor families such as MYB,NAC,WRKY,and bHLH showed significant differences.Joint analysis with metabolome data indicated that flavonoids,isoflavonoids,flavones,and flavonols,as well as differential genes and metabolites in the metabolites synthesis pathways,were significantly enriched during the color transition process of C.sinense‘Red Sun’.A total of 26 differentially expressed genes in the flavonoid metabolic pathway were identified,which all showed significant down-regulation during the transition from red to yellow leaves.Additionally,the contents of five differential metabolites were significantly decreased during this transition.During the transition from yellow to green leaves,17 genes and two metabolites(neohesperidin and naringin chalcone)showed significant differences.【Conclusion】The reduction in the content of anthocyanin synthesis precursors

关 键 词：墨兰 叶色变异 转录组 联合分析 相关性网络分析 

分 类 号：S682.31[农业科学—观赏园艺]